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Modifying Enzymes

Modifying Enzymes

Proteins that have the ability to structurally alter macromolecules without being altered themselves. Useful for a variety of molecular biology procedures; products include DNA and RNA polymerases, phosphatases, kinases, nucleases, etc.
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1 – 15 di 15 risultati
1

Fisher BioReagents™ Proteinase K (Tritirachium album/Molecular Biology)

Promozioni disponibili
Colore White
Content And Storage -20°C
Forma Solid
Sorgente Tritirachium album
Product Type Proteinase K
Da utilizzare con (applicazione) Isolation of DNA and RNA from cell and tissue preparations, inactivating many Enzymes, including Endogenous Nucleases
2

OPTIZYME™ T4 DNA Polymerase, Fisher BioReagents™

Colore Colorless
Content And Storage -20°C
Forma Liquid
Sorgente E. coli cells with a cloned gene 43 of bacteriophage T4
Product Type T4 DNA Polymerase
Da utilizzare con (applicazione) Synthesis of labeled DNA probes, creation of blunt PCR products with 3'-dA overhangs, ligation-independent cloning of PCR products, Restriction Enzymes, reverse transcriptases and T4 DNA ligase
pH 7.5
Tampone di conservazione OPTIZYME™ T4 DNA Pol Buffer 5X: Tris HCl 335mM (8.8 pH at 25°C), MgCl2 33mM, DTT 5mM, (NH4)2SO4 84mM
Concentrazione 5 U/μL
3

Fisher BioReagents™ Lysozyme, Egg White (White Cryst. Powder)

Colore White
Content And Storage <0°C
Forma Crystalline Powder
Sorgente Egg white
Product Type Lysozyme
Da utilizzare con (applicazione) Used to lyse E. coli for preparation of plasmid DNA from transformed cell cultures
7

OPTIZYME™ DNA Polymerase I, Fisher BioReagents™

Colore Colorless
Content And Storage -20°C
Forma Liquid
Sorgente E. coli cells with a cloned polA gene
Product Type DNA Polymerase I
Da utilizzare con (applicazione) DNA Polymerase I labels DNA by nick translation in conjunction with DNase I, second-strand cDNA synthesis in conjunction with RNase H, incorporation of modified Nucleotides, Reverse Transcriptases
pH 7.5
Tampone di conservazione OPTIZYME™ DNA Pol I Buffer 10X : Tris HCl 500mM (7.5 pH at 25°C), MgCl2 100mM, DTT 10mM
Concentrazione 10 U/μL
9

Fisher BioReagents™ Leupeptin Hemisulfate

(Acetyl-Leu-Leu-Arg-al), Valeramide

Peso molecolare 475.60
Colore White
Content And Storage -20°C
Forma Chunks, Powder
Product Type Leupeptin Hemisulfate
Da utilizzare con (applicazione) Inhibitor for Trypsin, Plasmin, Papain, Cathepsin B
10

OPTIZYME™ Alkaline Phosphatase, Fisher BioReagents™

Colore Colorless
Content And Storage -20°C
Forma Solid
Sorgente E. coli cells with a cloned bacterial Alkaline Phosphatase gene
Product Type Alkaline Phosphatase
Da utilizzare con (applicazione) DePhosphorylates cloning vector DNA (10 min. at 37°C), fast thermo-inactivation (5 min. at 75°C), Nucleotide degradation prior to sequencing PCR products, active on Nucleotides, Protein dephosphorylation, restriction En
pH 7.4
Tampone di conservazione OPTIZYME™ AP Buffer 10X : Tris HCl 100mM (8 pH at 37°C), MgCl2 50mM, KCl 1M, Triton™ X-100 0.2%, BSA 1mg/mL
Concentrazione 1 U/μL
12

OPTIZYME™ M-MLV Reverse Transcriptase, Fisher BioReagents™

Colore Colorless
Content And Storage -20°C
Forma Liquid
Sorgente E. coli cells with a cloned fragment of the pol gene encoding Moloney Murine Leukemia Virus Reverse Transcriptase
Product Type OPTIZYME™ M-MLV Reverse Transcriptase
Da utilizzare con (applicazione) Generation of labeled cDNA probes for microarrays, synthesis of cDNA for cloning and expression, analysis of RNA by primer extension, DNA labeling, optimum activity at 42°C, incorporates modified Nucleotides
pH 8.3
Tampone di conservazione OPTIZYME™ M-MLV RT Buffer 5X : Tris HCl 250mM (8.3 pH at 25°C), KCl 250mM, MgCl2 20mM, DTT 50mM
Concentrazione 200 U/μL
14

OPTIZYME™ T4 DNA Ligase, Fisher BioReagents™

Colore Colorless
Content And Storage -20°C
Forma Liquid
Sorgente E. coli cells with a cloned gene 30 of bacteriophage T4
Product Type T4 DNA Ligase
Da utilizzare con (applicazione) Used to clone restriction Enzyme generated DNA fragments, cloning PCR products, ligation of double-stranded Oligonucleotide linkers or adaptors to DNA, site-directed mutagenesis, amplified fragment length polymorphism, ligase-mediated RNA detection, nick
pH 7.5
Tampone di conservazione OPTIZYME™ T4 DNA Ligase Buffer 10X: Tris HCl 400mM, MgCl2 100mM, DTT 100mM, ATP 5mM (7.8 pH at 25°C), OPTIZYME™ PEG 4000 Solution 50%: Polyethylene Glycol 50% (w/v)
Concentrazione 5 U/μL