Thermo Scientific™ Colonne LC C18 Acclaim™ PepMap™ 100

Descrizione

Per la generazione di analisi a elevata risoluzione di peptidi triptici, naturali e di sintesi con colonne LC C18 Thermo Scientific™ Acclaim™ PepMap™ 100. Queste colonne vengono spesso impiegate nella mappatura dei peptidi LC-MS/MS per l’identificazione delle proteine, la ricerca dei biomarcatori e la biologia dei sistemi. Grazie all’elevata capacità di carico, le colonne C18 Acclaim PepMap 100 risultano particolarmente adatte per l’analisi di peptidi a bassa concentrazione in campioni proteomici complessi. La maggior parte delle colonne C18 è preassemblata con raccordi a serraggio manuale Thermo Scientific™ nanoViper™ per facilità di installazione. Sono disponibili anche colonne di tipo classico.

• High resolution in protein identification, biomarker discovery and systems biology
• Particular loadability allows hghest sensitivity in LC/MS
• Designed for TFA-free LC-MS, minimizing ion-suppression effects
• Optimized for coupling to ESI/MS and MALDI-MS
• Highest column-to-column reproducibility
• Easy-to-use, cutting-edge miniaturized HPLC
• Preassembled with nanoViper column inlet fittings for easy column installation

Key advantages of nanoViper fittings

• Ease of use, no sleeve for small capillaries
• Tool-free assembly
• No column damage from overtightening: capable of withstanding pressures up to 1000 bars and is compatible with third party valves and unions
• No experimental failure due to bad connections

• High loading capcity
• Use for the analysis of low abundant peptides often found in complex proteomics samples
• Select the column length and I.D. for your throughput, sensitivity and amount of sample available requirements
• Choose from 50, 150, 250, 500mm lengths and 50, 75, 300 and 1000μm I.D.
• Superior protein and peptide separations
• Trifluoroacetic acid (TFA)-free LC/MS minimizes suppression effects for enhanced MS sensitivity
• Outstanding separation efficiency
• Superior resolution and recovery
• Easy installation with nanoViper™ Fingertight Fittings

PepMap nano columns are also available pre-assembled with Thermo Scientific™ Dionex™ nanoViper™ Fingertight Fitting for a dead-volume-free connection to 1000 bar.

• Requires no tools
• Provides virtually zero-dead-volume connections
• Withstands pressures up to a 1000 bar

About Nano, capillary, and micro Acclaim PepMap columns

Nano (75μm), capillary (300μm) and micro (1000μm) columns are ideal for protein and peptide analyses. Sample amount is often limited and smaller I.D. columns can increase in sensitivity in sample-limited situations. While nano LC columns are often used in proteomics, the concentrating effects of capillary and micro columns are beneficial for metabolomics or for facilitating LC-MS interfacing. Thermo Scientific Acclaim PepMap columns are offered in nano, capillary, and micro column in a variety of chemistries for peptides and protein analysis.

Use PepMap Trap columns for desalting and preconcentration of peptides before LC separation with MS detection. The trap columns provide the highest efficiencies for one dimensional peptide mapping experiments and 2D-LC analyses.