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Applied Biosystems™ SYBR™ Green Universal Master Mix

Everything needed for SYBR™ Green dye-based PCR amplification and detection in a convenient, single-tube format.

Specifica

Metodo di rilevamento SYBR
Formato Tube
GC-Rich PCR Performance High
PCR Method qPCR
Polimerasi AmpliTaq Gold DNA Polymerase
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Includes: 2X mixture of SYBR Green I Dye, AmpliTaq Gold DNA Polymerase, dNTPs with dUTP, Passive Reference 1 (ROX), and optimized buffer components.

Descrizione

Descrizione

Applied Biosystems™ SYBR™ Green Universal Master Mix combines SYBR™ Green I dye, AmpliTaq Gold™ DNA Polymerase, dNTPs with dUTP, Passive Reference 1, and optimized buffer in the convenience of a single vial.

  • SYBR™ Green I dye detects double-stranded DNA, so specific probes are not required
  • AmpliTaq Gold™ DNA polymerase minimizes nonspecific product formation to achieve superior performance
  • dUTP significantly reduces carryover contamination when used in conjunction with uracil-DNA glycosylase
  • Proprietary buffer enhancements ensure performance and reliability
  • Premixed components stored at 2° to 8°C significantly reduce assay setup time

Maximum Flexibility and Convenience:

  • Applied Biosystems™ SYBR™ Green Universal Master Mix provides maximum flexibility at reduced cost because no target-specific TaqMan™ probes are required
  • SYBR™ Green I dye is a double-stranded DNA binding dye that detects any double-stranded DNA generated during PCR
  • The hot-start enzyme AmpliTaq Gold™ DNA Polymerase minimizes nonspecific product formation (including primer-dimers), yielding superior performance and sensitivity
  • Passive Internal Reference 1 is provided to normalize non-PCR—related fluorescence fluctuations
  • This minimizes well-to-well variability that can result from a variety of causes, such as pipetting error or sample evaporation
  • SYBR™ Green I dye is ideal for target identification (screening assays) or when a limited number of assays is needed.
  • Store at -20°C

Order Info

Shipping Condition: Wet Ice

Specifica

Specifica

SYBR
High
AmpliTaq Gold DNA Polymerase
SYBR Green
SYBR™
Gene Expression
Tube
qPCR
Standard
ROX (Pre-mixed)
Real Time PCR SYBR Master Mix
2X
Videos
SDS
Documenti

Documenti

Product Certifications

For Research Use Only. Not for use in diagnostic procedures.