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Applied Biosystems™ SYBR™ Green Universal Master Mix
Everything needed for SYBR™ Green dye-based PCR amplification and detection in a convenient, single-tube format.
Specifica
Metodo di rilevamento | SYBR |
---|---|
Formato | Tube |
GC-Rich PCR Performance | High |
PCR Method | qPCR |
Polimerasi | AmpliTaq Gold DNA Polymerase |
Includes: 2X mixture of SYBR Green I Dye, AmpliTaq Gold DNA Polymerase, dNTPs with dUTP, Passive Reference 1 (ROX), and optimized buffer components.
Descrizione
Applied Biosystems™ SYBR™ Green Universal Master Mix combines SYBR™ Green I dye, AmpliTaq Gold™ DNA Polymerase, dNTPs with dUTP, Passive Reference 1, and optimized buffer in the convenience of a single vial.
- SYBR™ Green I dye detects double-stranded DNA, so specific probes are not required
- AmpliTaq Gold™ DNA polymerase minimizes nonspecific product formation to achieve superior performance
- dUTP significantly reduces carryover contamination when used in conjunction with uracil-DNA glycosylase
- Proprietary buffer enhancements ensure performance and reliability
- Premixed components stored at 2° to 8°C significantly reduce assay setup time
Maximum Flexibility and Convenience:
- Applied Biosystems™ SYBR™ Green Universal Master Mix provides maximum flexibility at reduced cost because no target-specific TaqMan™ probes are required
- SYBR™ Green I dye is a double-stranded DNA binding dye that detects any double-stranded DNA generated during PCR
- The hot-start enzyme AmpliTaq Gold™ DNA Polymerase minimizes nonspecific product formation (including primer-dimers), yielding superior performance and sensitivity
- Passive Internal Reference 1 is provided to normalize non-PCR—related fluorescence fluctuations
- This minimizes well-to-well variability that can result from a variety of causes, such as pipetting error or sample evaporation
- SYBR™ Green I dye is ideal for target identification (screening assays) or when a limited number of assays is needed.
- Store at -20°C
Order Info
Shipping Condition: Wet Ice
Specifica
SYBR | |
High | |
AmpliTaq Gold DNA Polymerase | |
SYBR Green | |
SYBR™ | |
Gene Expression |
Tube | |
qPCR | |
Standard | |
ROX (Pre-mixed) | |
Real Time PCR SYBR Master Mix | |
2X |
For Research Use Only. Not for use in diagnostic procedures.