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IBA Lifesciences Avidin
Avidin can efficiently block of biotin and biotinlyated proteins.
Marca: IBA Lifesciences 2-0204-050
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Descrizione
Avidin is a tetrameric biotin binding protein (KD ∼1 x 10-14) extracted from egg white. It prevents biotin and biotinylated proteins from binding to Strep-Tactin™ or Strep-Tactin™XT, whereas binding of the Twin-Strep-tag™ and Strep-tag™II is not influenced.Biotin and/or biotinylated proteins can potentially reduce the binding capacity of Strep-Tactin™ as well as Strep-Tactin™XT and are present in cell lysates and cell culture media, especially from mammalian or insect cells. Thus, if Twin-Strep-tag™ and Strep-tag™II fusion proteins from biotin-containing extracts or media are intended to be purified via Strep-Tactin™ or Strep-Tactin™XT affinity chromatography, biotin has to be masked by addition of avidin prior to application onto the resin.
Specifica
| 50 mg |
For Research Use Only.