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BD Human IFN-γ ELISA Kit II
For in vitro quantitative determination of human interferons in serum, plasma, and cell culture samples
Marca: BD 550612
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Descrizione
The OptEIA™ test is a solid phase sandwich ELISA (Enzyme-Linked Immunosorbent Assay).
- Reactivity: Human
- Utilizes monoclonal antibody specific for IFN-γ coated on a 96-well plate
ELISA
Includes:
- Antibody Coated Wells: 2 plates of 96 breakable wells (12 strips x 8 wells) coated with anti-human IFN-Γ monoclonal antibody
- Detection Antibody: 30mL of biotinylated anti-human IFN-Γ monoclonal antibody with ProClin™-150 as preservative
- Standards: 4 vials lyophilized recombinant human IFN-Γ
- Enzyme Concentrate (250x): 150μL of 250x concentrated Streptavidin-horseradish peroxidase conjugate with BSA and ProClin-300 as preservative
- Standard/Sample Diluent: 30mL of animal serum with 0.09% sodium azide as preservative
- ELISA Diluent: 12mL of a buffered protein base with 0.09% sodium azide as preservative
- Wash Concentrate (20x): 100mL of 20x concentrated detergent solution with ProClin-150 as preservative
- TMB One-Step Substrate Reagent: 30 ml of 3,3',5,5'-tetramethylbenzidine (TMB) in buffered solution
- Stop Solution: 13mL of 1 M phosphoric acid
- Plate Sealers: 4 sheets with adhesive backing
Specifica
| IFN | |
| Human IFN-g Antibody coated plate, detection Antibody biotin Anti-Human IFN-g, Human IFN-gamma standard, TMB one-step Substrate reagent, Enzyme concentrate Streptavidin -HRP, wash concentrate (20X), ELISA Diluent, stop solution, standard/Sample Diluent | |
| Serum, Plasma, Cell Culture | |
| In-vitro quantitative determination of Human interferons, ELISA |
| Human | |
| 2 Plates | |
| 2°C to 8°C | |
| Human IFN-gamma ELISA kit II |
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Research Use Only
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