Thermo Scientific™ Imject™ Blue Carrier™ Protein

Descrizione

Thermo Scientific™ Blue Carrier Protein is a highly-soluble, mollusk-derived hemocyanin formulated in convenient package sizes and a complete kit for carbodiimide (EDC-mediated) hapten-carrier conjugation via amine and carboxyl groups.

Highlights:

• Convenient packages – choose lyophilized Blue Carrier Protein or complete EDC conjugation kit

• High-yield conjugation – each molecule of CCH contains hundreds of primary amines available for coupling haptens via EDC or NHS ester crosslinkers

• Validated quality – the immunogenic carrier protein is specially purified to ensure uniform chemical structure and solubility for consistent performance

• Superior solubility – increased solubility compared to KLH allows Blue Carrier Protein to be used in a broader range of conjugation protocols to accommodate unusual or poorly soluble haptens

• Highly immunogenic – provides essentially the same level of immunogenicity and phylogenetic distance as keyhole limpet hemocyanin (KLH)

Properties of Blue Carrier Protein:

• Alternative names: Concholepas concholepas hemocyanin, CCH
• Molecular weight: Whole molecule: ∽8,000,000; Subunits: ∽400,000 and ∽350,000
• Absorption: maxima 280nm, 350nm and 558nm
• Extinction coefficient (Abs. 1mg/mL solution): 1.4 at 280nm; 0.35 at 346nm
• A280/A350 Ratio: ∽4.0
• Copper/protein ratio: ∽16 Cu²⁺/protein molecule
• Isoelectric point: 6.0
• Solubility: Soluble in aqueous buffers pH 7.0 to 8.0
• Stability: Stable in aqueous buffers (∽pH 7.2 without Ca²⁺ or Mg²⁺)

Includes:

Kit contains lyophilized Blue Carrier Protein (in MES buffer), EDC crosslinker, buffers and desalting columns to easily prepare ready-to-use immunogens for injection