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Thermo Scientific™ EZ-Link™ Psoralen-PEG3-Biotin
Biotinylate DNA or RNA via intercalation and conjugation with thymine- and other pyrimidine-containing bases with this light-activated biotinylation reagent.
Marca: Thermo Scientific™ 29986
Dettagli aggiuntivi : CAS : 175201-86-2 Peso : 0.05000kg
Descrizione
Thermo Scientific™ EZ-Link Psoralen-PEG3-Biotin enables biotinylation of DNA or RNA via UV-light-activated intercalation of the psoralen group with thymine- and other pyrimidine-containing bases to form covalent bonds.
Highlights:
- Water-soluble, polyethylene glycol (PEG) spacer arm
- Much higher coupling yield than achieved with photoactivatable biotin
- Psoralen moiety reacts through a cyclo-addition mechanism with the 5,6 double bond in thymine- and other pyrimidine-containing bases
- Covalent attachment occurs with exposure to UV light (approximately equal to 350nm)
- Great for biotinylating DNA/RNA probes (∽1 biotin/10-20 bases)
- Ultrahigh sensitivity relative to radiolabeled probes (<100 femtograms)
- Does not interfere with hybridization
Specifica
Psoralen-PEG3-Biotin | |
Store at 4°C protected from light and moisture. Shipped at ambient temperature. | |
Cell-Impermeant | |
Psoralen | |
Biotin | |
Biotin & Analogs |
5 mg | |
EZ-Link™ | |
Light-activated | |
DMF (Dimethylformamide), DMSO (Dimethylsulfoxide), Water | |
Extra-long, Pegylated |
For Research Use Only. Not for use in diagnostic procedures.