Thermo Scientific™ DNA polimerasi DreamTaq™

Descrizione

Perform all standard PCR applications with Thermo Scientific™ Fermentas DreamTaq™ DNA Polymerase, an enhanced Taq DNA polymerase.

• Robust amplification with minimal optimization
• High yields of PCR products
• Higher sensitivity compared to conventional Taq DNA polymerase
• Amplification of long targets up to 6kb from genomic DNA and up to 20kb from viral DNA
• Generates 3'-dA overhangs
• Incorporates modified nucleotides

Definition of Activity Unit

• One unit of the enzyme catalyzes the incorporation of 10nmol of deoxyribonucleotides into a polynucleotide fraction (adsorbed on DE-81) in 30 min at 70°C
• Enzyme activity is assayed in the following mixture: 67mM Tris-HCl (pH 8.8 at 25°C), 6.7 mM MgCl₂, 1mM 2-mercaptoethanol, 50mM NaCl, 0.1mg/mL BSA, 0.75mM activated calf thymus DNA, 0.2mM of each dNTP, 0.4MBq/mL [3H]-dTTP

Storage Buffer

• The enzyme is supplied in: 20mM Tris-HCl (pH 8.0), 1mM DTT, 0.1mM EDTA, 100mM KCl, stabilizing agent and 50% (v/v) glycerol

Quality Control

• The absence of endo-, exodeoxyribonucleases and ribonucleases confirmed by appropriate quality tests; Functionally tested in PCR

Inhibition and Inactivation

• Inhibitors: ionic detergents (deoxycholate, sarkosyl and SDS) at concentrations higher than 0.06, 0.02 and 0.01%, respectively (1)
• Inactivated by phenol/chloroform extraction

Recommended for:

Routine PCR amplification of DNA fragments up to 6kb; RT-PCR; Genotyping; Generation of PCR products for TA cloning