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Fisher BioReagents™ Taq DNA Polymerase
Allows up to 100 cycles of amplification without loss of activity
Marca: Fisher BioReagents™ FB6000-20
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Descrizione
- Licensed for use in PCR
- Uses a thermostable DNA polymerase that has been encoded by a modified form of Thermus aquaticus DNA polymerase gene that has been inserted into an E. coli host
- Has a 5«→3« exonuclease activity
- Available with two different reaction buffers
- Buffer A contains an optimized final concentration of 15mM MgCl2 for most PCR amplifications
- Buffer B does not contain MgCl2; MgCl2 is provided in a separate vial as a 25mM solution
- Storage Buffer: 20mM Tris-HCl, pH 7.5 (at room temperature), 100mM KCl, 0.1mM EDTA, 1mM DTT, 50% Glycerol, 0.5% Tween™ 20, 0.5% Nonidet P40™
- Recommended Assay Buffer: 10X Assay Buffer A (100mM Tris-HCl, pH 9.0 [at 25°C/77°F]; 500mM KCl; 15mM MgCl2) or 10X Assay Buffer B (100mM Tris-HCl, pH 9.0 [at 25°C/77°F]; 500mM KCl) and 25mM MgCl2 solution in a separate vial
Unit Definition: One unit of enzyme is defined as the amount that will incorporate 10nmol of dNTPs into acid-insoluble material per 30 minutes in a 10-minute incubation at 74°C (165°F) under the assay conditions above
Specifica
| 10X assay buffer A : Tris-HCl 100mM, pH 9 at 25°C, KCl 500mM and MgCl2 15mM solution in a separate vial | |
| Taq DNA Polymerase | |
| Poly Tube | |
| Standard PCR | |
| Tris-HCl 20mM, pH 7.5 (at room temperature), KCl 100mM, EDTA 0.1mM, DTT 1mM, Glycerol 50%, Tween™ 20 0.5%, Nonidet™ P40™ 0.5% |
| -20°C | |
| Liquid | |
| 25 × 100 Units | |
| 5U/μL |