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Fisher BioReagents™ SurePrep™ Nuclear or Cytoplasmic RNA Purification Kit
Rapid method for the isolation and purification of both nuclear and cytoplasmic RNA from cultured animal cells and animal tissue samples
Marca: Fisher BioReagents™ BP2805-50
Includes: Lysis solution (20mL); Wash solution (22mL); Binding solution (25mL); RNA elution solution (6mL); Spin columns (50); Collection tubes (50); Elution tubes (1.7mL) (50); Product insert (1)
Descrizione
Rapid method for the isolation and purification of both nuclear and cytoplasmic RNA from cultured animal cells and animal tissue samples
- Fast and easy processing using rapid spin-column format
- High quality intact RNA can be used in various downstream applications
- Enrich for cytoplasmic or nuclear RNA depending on downstream application
- No genomic DNA contamination in the cytoplasmic fraction
- No phenol or chloroform extractions
- Isolate all sizes of RNA from each fraction, including all small RNA molecules (>200 nt)
- Kit provides sufficient reagents to perform either 50 cytoplasmic RNA preparations or 25 cytoplasmic and nuclear preparations
Applications:
Purification of Nuclear and Cytoplasmic RNA suitable for real-time PCR, reverse transcription PCR, Northern blotting, RNase protection and primer extension, and expression array assays.
Specifica
| Spin Column | |
| Lysis solution 20mL, wash solution 22mL, binding solution 25mL, RNA elution solution 6mL, spin columns 50, collection tubes 50, elution tubes 1.7mL 50, product insert 1 50μg Column Binding Capacity, 600μL Max. column lo | |
| 35μg Grape leaves (50mg), 32μg plum leaves (50mg), 60μg tobacco leaves (50mg), 60μg tomato leaves (50mg) |
| RT-PCR, reverse transcription PCR, northern blotting, RNase protection and primer extension and expression array assays | |
| 75 Preps |