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Fisher BioReagents™ SurePrep™ Small RNA Purification Kit
Optimized for enrichment of small RNA by removing large RNA for studying regulation of gene expression
Marca: Fisher BioReagents™ BP2801-25
Includes: Lysis solution (2 x 20mL), Wash solution (22mL), RNA elution buffer (6mL), Large RNA removal columns (25), Small RNA enrichment columns (25), Collection tubes (50), Elution tubes (1.7mL) (50) and Product insert
Descrizione
- Isolate all small RNA molecules (<200 nucleotides)
- Minimal contamination from large RNA molecules and genomic DNA
- Fast and easy processing using rapid spin-column format
- No phenol or chloroform extractions
- High quality small RNA such as miRNA and siRNA can be used in various downstream applications
Applications:
Purified small RNA is suitable for use in RT-PCR, Northern blotting and microarray analysis
Specifica
| Spin Column | |
| Lysis solution 2 x 20mL, wash solution 22mL, RNA Elution Buffer 6mL, large RNA removal columns 25, small RNA enrichment columns 25, collection tubes 50, elution tubes 1.7mL 50 and product insert | |
| 15μg HeLa cells (1 x 106 cells), 50μg E. coli (1 x 109 cells) |
| RT-PCR, northern blotting and microarray analysis | |
| 25 Preps |
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