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Novus Biologicals™ FoxP3
Foxp3, human (full length cDNA) in PCLXSN Retroviral expression vector
Marca: Novus Biologicals™ NBP2-29545
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Descrizione
- RetroMax system takes advantages of two properties of 293 cells (high level of transfectibility and strong E1A-mediated stimulation of CMV promoter controlled transcription)
- 293 cells of nonmurine origin avoid problem of selective packaging and transfer of VL30 genomes (present in all murine packaging cells)
- Vector supernatants are free of helper virus and are of sufficiently high titer within 2 days of transient transfection in 293 cells
- For amplification, transform DH-5alpha or XL-1 Blue and plate on LB plates containing 50mg/mL ampicillin
- Buffer: 20μL 1x TE (10mM Tris, pH 7.5, 1mM EDTA)
Recommended Storage
Store at -20°C. Avoid freeze-thaw cycles.
Specifica
| 20µL 1x TE (10mM Tris, pH 7.5, 1mM EDTA) | |
| Store at -20°C. Avoid freeze-thaw cycles. | |
| 2 μg | |
| FoxP3 Vector |
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