Directly detect histidine-tagged fusion proteins and other histidine-rich proteins with this nickel-activated horseradish peroxidase (HRP) derivative.
Marca: Thermo Scientific™ 15165
278.16 EUR valido fino al 2025-04-18
Usa il codice promo "24107" per avere il tuo prezzo promozionale.
Thermo Scientific™ HisProbe-HRP is an nickel (Ni2+)-activated derivative of horseradish peroxidase (HRP). This product has been optimized for direct detection of recombinant histidine-tagged proteins and other histidine-rich proteins.
Unlike anti-6xHis antibodies, HisProbe-HRP can recognize polyhistidine tags independent of adjacent tags. The detection kit includes blocking buffers and chemiluminescent substrate for sensitive Western blot detection via the HisProbe-HRP Conjugate. The active ligand in HisProbe-HRP is a tridentate chelator that allows Ni2+ to be bound in active form for subsequent interaction and detection fo target molecules. The active chelator has binding capabilities similar to iminodiacetic acid (IDA) when used for immobilized metal affinity chromatography (IMAC).Highlights:
Fast – His-tag detection is achieved with a one-step probe incubation that eliminates the need to run a lengthy two-step primary/secondary antibody reaction protocol
Economical – no need to purchase expensive primary/secondary antibodies; considerably less costly than anti-6xHis-tag antibodies
High specificity – HisProbe-HRP can recognize his-tags independent of adjacent tags
Less steric hindrance – HisProbe-HRP is smaller than antibodies and detects his tagged fusion proteins that are often undetectable when using some anti-his-tag antibodies
| Coniugato HisProbe-HRP Pierce | |
| Chemiluminescence | |
| HisProbe™ | |
| All species |
| HRP Conjugate | |
| Solid | |
| 2 mg | |
| Storage: Upon receipt store product at 4°C. Product is shipped at ambient temperature. |
Per poter ottenere i certificati è necessario indicare un numero di lotto. Per individuare il numero di lotto su ordini precedenti usi l'area dedicata allo stato degli ordini.
| Numero di lotto | Tipo di certificato | Data | Catalog Number |
|---|---|---|---|
| ZJ392290 | Certificato di analisi | 25/10/2024 | 15165 |
| ZD394415 | Certificato di analisi | 26/04/2024 | 15165 |
| ZA384963 | Certificato di analisi | 31/01/2024 | 15165 |
| YH376624 | Certificato di analisi | 24/08/2023 | 15165 |
| YC368392A | Certificato di analisi | 14/04/2023 | 15165 |
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For Research Use Only. Not for use in diagnostic procedures.
