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Thermo Scientific™ Agarosio HisPur™ Ni-NTA Superflow
Perform FPLC purification of poly-histidine-tagged proteins with this nitrilotriacetic acid (NTA) modified Superflow 6 support charged with divalent nickel (Ni+2).
Marca: Thermo Scientific™ 25217
8797.00 EUR valido fino al 2024-12-31
Usa il codice promo "21963" per avere il tuo prezzo promozionale.
Dettagli aggiuntivi : Peso : 0.01000kg
Descrizione
Perform FPLC purification of poly-histidine-tagged proteins with Thermo Scientific™ HisPur Ni-NTA Superflow Agarose, a nitrilotriacetic acid (NTA) modified Superflow 6 support charged with divalent nickel (Ni+2).
Additional Information and Online Ordering
HisPur Ni-NTA Superflow Agarose is a highly crosslinked, durable resin that does not compress at flow rates used for medium- or large-scale FPLC purifications. The resin holds up well to a variety of chemicals and pH values, and is compatible with common clean-in-place procedures. Compared to cobalt and other ligands used for immobilized metal affinity chromatography (IMAC), nickel provides greater capacity for His-tagged protein purification. HisPur Ni-NTA Superflow Agarose exhibits a high dynamic binding capacity across a range of flow rates, making it an excellent choice for larger scale purifications.Highlights:
High capacity – binds greater than 20mg of His-tagged GFP per mL of resin at a linear flow rate of 300cm/hour
High purity – provides greater than 80% purity of eluted fractions when used to purify from lysates
Versatile – can be used to purify proteins under native and denaturing conditions
Robust – highly crosslinked beads tolerate linear flow rates up to 1200cm/hour
Compatible – maintains function in a wide variety of chemicals and pH conditions
Cost effective – competitively priced resin is stable through multiple cycles of cleaning and reuse
Recommended for:
Medium- to large-scale FPLC purification of polyhistidine tagged proteins
Specifica
20 mg His-Tagged GFP Binding per mL Resin | |
Liquid Suspension | |
1 L | |
Agarose | |
Bottle |
Store at 4°C | |
HisPur™ | |
His-Tagged Protein | |
Divalent Nickel, Metal Chelate | |
Affinity, Agarose Resin |
For Research Use Only. Not for use in diagnostic procedures.