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  7. 13414199

Thermo Scientific™ Agarosio HisPur™ Ni-NTA Superflow

Perform FPLC purification of poly-histidine-tagged proteins with this nitrilotriacetic acid (NTA) modified Superflow 6 support charged with divalent nickel (Ni+2).

Marca:  Thermo Scientific™ 25217

8797.00 EUR valido fino al 2024-12-31
Usa il codice promo "21963" per avere il tuo prezzo promozionale.


Dettagli aggiuntivi : Peso : 0.01000kg

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Codice prodotto. 13414199

  • 9260.00€ / 1L
Spedizione stimata: 06-08-2024
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Descrizione

Descrizione

Perform FPLC purification of poly-histidine-tagged proteins with Thermo Scientific™ HisPur Ni-NTA Superflow Agarose, a nitrilotriacetic acid (NTA) modified Superflow 6 support charged with divalent nickel (Ni+2).


Additional Information and Online Ordering »

HisPur Ni-NTA Superflow Agarose is a highly crosslinked, durable resin that does not compress at flow rates used for medium- or large-scale FPLC purifications. The resin holds up well to a variety of chemicals and pH values, and is compatible with common clean-in-place procedures. Compared to cobalt and other ligands used for immobilized metal affinity chromatography (IMAC), nickel provides greater capacity for His-tagged protein purification. HisPur Ni-NTA Superflow Agarose exhibits a high dynamic binding capacity across a range of flow rates, making it an excellent choice for larger scale purifications.

Highlights:

  • High capacity – binds greater than 20mg of His-tagged GFP per mL of resin at a linear flow rate of 300cm/hour

  • High purity – provides greater than 80% purity of eluted fractions when used to purify from lysates

  • Versatile – can be used to purify proteins under native and denaturing conditions

  • Robust – highly crosslinked beads tolerate linear flow rates up to 1200cm/hour

  • Compatible – maintains function in a wide variety of chemicals and pH conditions

  • Cost effective – competitively priced resin is stable through multiple cycles of cleaning and reuse

Recommended for:

Medium- to large-scale FPLC purification of polyhistidine tagged proteins

Specifica

Specifica

20 mg His-Tagged GFP Binding per mL Resin
Liquid Suspension
1 L
Agarose
Bottle
Store at 4°C
HisPur™
His-Tagged Protein
Divalent Nickel, Metal Chelate
Affinity, Agarose Resin
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For Research Use Only. Not for use in diagnostic procedures.