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Cytiva Colonne preimpaccate HisTrap™ excel
Per la cattura e la purificazione di proteine marcate con istidina, secrete in surnatanti di colture cellulari eucariotiche. Le colonne preimpaccate Cytiva Lifescience™ HisTrap™ excel sono progettate per purificazioni pratiche e rapide.
Marca: Cytiva 29048586
Dettagli aggiuntivi : CAS : 64-17-5 Peso : 0.10200kg
Descrizione
The design of the columns in combination with the specific properties of the medium enables fast and convenient purifications.
- Ready-to use prepacked with Ni Sepharose excel
- Special type of filter in top and bottom of the columns to load large volumes of cell-free, un clarified samples directly on the columns helps prevent degradation and loss of sensitive target proteins
- Made of biocompatible polypropylene and cannot be opened or refilled
- Delivered with stopper on inlet and snap-off end on outlet
- Operated either with peristaltic pump or with chromatography systems such as KTA systems
- Binding Capacity/Column: At least 10 mg histidine-tagged protein/ml sedimented medium
- Average particle size: 90μm
- Matrix: Highly cross-linked agarose, 6%
- pH Stability working range: 2 to 12
- pH Stability cleaning range: 2 to 14
- Flow velocity: 150 cm/h - 600 cm/h4
- Column I.D.: 7 mm
HisTrap excel 1mL column
- Bed Dimensions: 7 × 25mm
- Flow rate: 1 to 4mL/min.
- Bed Dimensions: 16 × 25mm
- Flow rate: 5 to 20mL/min.
Specifica
1 mL | |
7 mm | |
1 to 4 mL/min. | |
2 to 12 | |
4°C to 30°C | |
1 mL |
0.2 in. | |
HisTrap excel columns are prepacked with Ni Sepharose excel affinity resins for capture and purification of histidine-tagged proteins secreted into eukaryotic cell culture supernatants by immobilized metal ion affinity chromatography (IMAC). | |
Polypropylene | |
6% Highly Cross-linked Agarose | |
5 bar (0.5 MPa, 70 psi) | |
Pre-packed Column |
Sicurezza e movimentazione
- Attenzione