Thermo Scientific™ Imject™ Purification Buffer Salts

Descrizione

Thermo Scientific™ Imject Mariculture Keyhole Limpet Hemocyanin (mcKLH) is formulated to achieve optimum performance with different conjugation chemistries, and includes a complete kit for carbodiimide-mediated hapten-carrier preparation.

Mariculture KLH (mcKLH) is purified and lyophilized in buffers that optimize its stability and solubility for hapten conjugation. The large, complex protein contains hundreds of sites per molecule for effective conjugation of peptides and other antigens using amine-reactive or carboxyl-reactive crosslinkers.

Preparations in phosphate buffer are ready for hapten-carrier conjugation via amine-reactive NHS-ester or glutaraldehyde crosslinking. Preparations in MES buffer are optimized for peptide-carrier conjugation via the carboxyl-reactive carbodiimide crosslinker called EDC (or EDAC). The Imject EDC Spin Kit includes ready-to-use units of KLH carrier protein, optimized reaction buffers and trouble-free spin desalting columns for immunogen preparation using the EDC method.

Highlights:

• Convenient packages – choose lyophilized KLH protein or complete EDC conjugation kit

• High-yield conjugation – each molecule of KLH contains hundreds of primary amines available for coupling haptens via EDC or NHS ester crosslinkers

• Validated quality – purified and stabilized mariculture KLH maintains solubility in aqueous solutions, unlike traditional sources of the carrier protein

• Sustainable source – KLH is harvested from select populations of the mollusk Megathura crenulata (keyhole limpet) that are grown in mariculture, not extracted from wild populations

• Highly immunogenic – KLH has a high molecular mass (0.5 to 13 million daltons; aggregates of 350 and 390kDa subunits) and elicits a stronger immune response than BSA or ovalbumin