Applied BioSystems™ MagMAX™ Saliva gDNA Isolation Kit

Descrizione

The MagMAX Saliva gDNA Isolation Kit is a scalable and automatable magnetic bead-based sample preparation technology for the isolation of genomic DNA (gDNA) from fresh and stabilized saliva. The resulting purified gDNA is ideal for a variety of downstream molecular biology applications such as qPCR, next-generation sequencing, and microarray analysis. All components are also available for purchase separately.

Key features of the MagMAX Saliva gDNA Isolation Kit:
• Process up to 96 samples in 25 minutes with 5 minutes hands-on time
• Isolate gDNA from 200 μL–2 mL of saliva using simple protocols in SBS microtiter plates
• Skip sample and buffer volume normalization steps for hassle-free processing
• Download validated BindIt software automation scripts for KingFisher Duo Prime and KingFisher Flex purification systems

Isolate DNA from stabilized and freshly collected saliva
The MagMAX Saliva gDNA Isolation Kit isolates gDNA from 200 μL–2 mL of freshly collected saliva or stabilized saliva collected using a commercially available saliva collection device (see figure below). MagMAX Saliva gDNA isolation chemistry is compatible with the Oragene™ saliva collection devices from DNA Genotek, the SalivaGard™ saliva collection device from Biomatrica, and the GeneFiX™ Saliva DNA Collection Device from IsoHelix.

The MagMAX Saliva gDNA Isolation Kit maximizes gDNA yields by treating samples with an innovative lysis/binding solution to simultaneously lyse cells, denature proteins, and capture gDNA on high-capacity magnetic binding beads. The Saliva gDNA Wash I Solution removes proteins, detergents, and other impurities from the bound nucleic acid. The gDNA is then released from the beads using a low-TE elution solution.

Fast and hassle-free magnetic bead-based workflows
The MagMAX Saliva gDNA Isolation Kit combines multiple laborious sample processing steps together into a single step to minimize the time and effort typically required by other methods, without compromising yield and purity. Process a broad range of sample volume inputs simultaneously without sample normalization or reagent volume adjustments for hassle-free sample prep. Simply match your saliva input volumes to one of three standard input workflows (50 μL to 500 μL, up to 1 mL, up to 2 mL) and follow the instructions for semi-automated sample preparation using the KingFisher Duo Prime or KingFisher Flex purification systems.

Optimized for KingFisher purification systems
The MagMAX Saliva gDNA Isolation Kit is optimized for use on the moderate-throughput KingFisher Duo Prime (Cat. No. 5400110) and the higher-throughput KingFisher Flex (Cat. No. 5400630) purification systems. Users can process up to 500 μL of saliva in high-throughput capacity SBS 96-well deep-well microtiter plates using the KingFisher Flex system. For smaller sample batches, users can process 12 samples using the Duo Prime system. Larger sample input volumes (>500 μL, and 1–2 mL), which are more challenging to process, are purified in the larger sample volume capacity 24-well deep-well SBS plates for 24 samples on the Flex or 6 samples on the Duo Prime system. KingFisher instrument BindIt software scripts for all sample types and both Flex and Duo Prime systems can be downloaded below and installed onto the KingFisher instruments.