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Thermo Scientific™ Kit di estrazione nucleare e citoplasmatica NE-PER™
Efficiently lyse cells and extract separate cytoplasmic and nuclear protein fractions in less than two hours with this subcellular fractionation kit.
Marca: Thermo Scientific™ 78833
325.65 EUR valido fino al 2024-08-31
Usa il codice promo "21743" per avere il tuo prezzo promozionale.
Dettagli aggiuntivi : Peso : 0.80000kg
Descrizione
Thermo Scientific™ NE-PER Nuclear and Cytoplasmic Extraction Kit provides for efficient cell lysis and extraction of separate cytoplasmic and nuclear protein fractions in less than two hours.
The NE-PER Kit is a nuclear protein extraction method that involves simple, stepwise lysis of cells and centrifugal isolation of nuclear and cytoplasmic protein fractions. A benchtop microcentrifuge, tubes and pipettors are the only tools required. The NE-PER Reagents efficiently solubilize and separate cytoplasmic and nuclear proteins into fractions with minimal cross-contamination or interference from genomic DNA and mRNA. Once desalted or diluted, the isolated proteins can be used to perform immunoassays and protein interaction experiments, such as mobility shift assays (EMSA), co-immunoprecipitation (Co-IP) and pull-down assays.
Highlights:
- Fast – obtain nuclear and cytoplasmic fractions in less than two hours
- Proven – the NE-PER Kit is referenced in more than 950 distinct publications
- Scalable – two kit sizes for producing extracts from cells and tissues
- Convenient – simple instructions do not require ultracentrifugation over gradients
- Compatible – use for downstream assays, including Western blotting, gel-shift assays, protein assays, reporter gene assays and enzyme activity assays
Includes:
Two kit sizes, each containing three buffer and detergent solutions
Compatible with:
Thermo Scientific LightShift Chemiluminescent EMSA Kit (Mfr. No. 20148)
Storage Requirements:
Upon receipt store kit components at 4°C
Specifica
| Nuclear and Cytoplasmic Extraction Reagent | |
| Ambient | |
| NE-PER™ | |
| Cell Lysis Buffer, Detergent Solution, Subcellular Fractionation |
| Upon receipt store kit components at 4°C. | |
| Liquid | |
| Lysis Buffer | |
| Kit da 15 ml |
Sicurezza e movimentazione
Identificativo prodotto
Avvertenza
Categoria di pericolo
Indicazioni di pericolo
Consigli di prudenza
Informazioni supplementari
- NE-PER Nuclear and Cytoplasmic Extraction Reagents
Avvertenza
- Attenzione
Categoria di pericolo
- Corrosione/irritazione della pelle Categoria 2
- Lesione oculare grave/irritazione oculare Categoria 2
Indicazioni di pericolo
- H315-Provoca irritazione cutanea.
- H319-Provoca grave irritazione oculare.
Consigli di prudenza
- P280-Indossare guanti/indumenti protettivi/Proteggere gli occhi/il viso.
- P264-Lavare accuratamente dopo l'uso.
- P305+P351+P338-IN CASO DI CONTATTO CON GLI OCCHI: sciacquare accuratamente per parecchi minuti. Togliere le eventuali lenti a contatto se è agevole farlo. Continuare a sciacquare.
Informazioni supplementari
- MIXTURE LIST-Contiene: Non-ionic Detergent,Salt
For Research Use Only. Not for use in diagnostic procedures.