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Thermo Scientific™ North2South™ Chemiluminescent Hybridization and Detection Kit
Acquire consistent, highly sensitive Southern blot results with a novel ECL HRP substrate and optimized hybridization and blocking buffers.
Marca: Thermo Scientific™ 37555
124.00 EUR valido fino al 2024-12-14
Usa il codice promo "21632" per avere il tuo prezzo promozionale.
Allerta:
To receive the discount customers must purchase three of the same product at list price in a single order to receive 33% discount. There is no limit to the multiples of 3 that customers can buy. Use promo code ”21632” to get your promotional price
Descrizione
Thermo Scientific™ North2South Chemiluminescent Detection Kit combines a novel ECL substrate for horseradish peroxidase (HRP) and optimized hybridization and blocking buffers to ensure consistent, high-sensitivity Southern blot results.
The Thermo Scientific North2South Hybridization and Detection Kit combines a novel enhanced luminol substrate for horseradish peroxidase (HRP) with optimized pre-made hybridization and blocking buffers to ensure consistent results and high sensitivity. Any nucleic acid blot can be hybridized with the appropriate biotinylated (biotin-labeled) oligonucleotide probe. After a brief membrane blocking step, the biotin label is detected with streptavidin-HRP and a 5-minute incubation with the chemiluminescent substrate. Results are easily recorded by brief exposure to X-ray film (1-15 minutes) or CCD camera.Highlights:
- Sensitivity equal to or greater than 32P
- Amenable to many formats
- High level of light output and consistent low background allows for very short exposure times using cooled CCD camera systems or film
- Ready-to-use buffers and short process time makes switching from radioactive detection to chemiluminescent detection an easy transition, even for the novice user
Specifica
| Yields 60mM sodium citrate, 600mM sodium chloride, 0.2% SDS, pH 7.9 when diluted 1:1 in water | |
| Non-radioactive Southern and Northern blotting | |
| North2South Hybridization Stringency Wash Buffer (2X) | |
| 375 mL |