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Invitrogen™ PureLink™ RNA Mini Kit
The PureLink RNA Mini Kit is a column-based kit used to isolate high-quality total RNA from a wide variety of sample types in 20 minutes using standard laboratory equipment.
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Specifica
| Isolation Technology | Silica Spin Column |
|---|---|
| Purification Time | 20 min. (After sample homogenization) |
| Volume di eluizione | 30–300 μL |
| Tipo di campione | Bacteria, Blood, Cells, Plant, Tissue, Yeast, Enzymatic Reactions |
| Tipo di prodotto finale | Total RNA |
Descrizione
Rapid and convenient column purification
The PureLink™ RNA Mini Kit provides rapid purification of total RNA from a wide range of cells and tissue types to yield up to 1000 μg of purified RNA from a single extraction. High-quality total RNA can be obtained from mini- to midi-prep amounts of starting material with no genomic DNA contamination.
Simplified, nontoxic RNA purification
The PureLink™ RNA Mini Kit combines nontoxic guanidine-isothiocyanate lysis with the speed, purity, and ease-of-use of silica-membrane purification. The safe and easy procedure can typically be completed in less than 20 minutes without the need for hazardous phenol/chloroform extraction, CsCl centrifugation, LiCl, or alcohol precipitation.
- Obtain high-yield, high-integrity RNA
- Rapid, non-hazardous RNA purification capabilities
- Ready-to-use RNA for many downstream applications
Specifica
| Silica Spin Column | |
| 30–300 μL | |
| Total RNA | |
| RT-PCR, qPCR, cDNA Library Construction, NGS, Microarray Analysis, Blot Hybridization, Northern Blotting, In Vitro Translation, Nuclease Protection Assays, Nucleic Acid Labeling | |
| Room Temperature | |
| Up to 350 μg |
| 20 min. (After sample homogenization) | |
| Bacteria, Blood, Cells, Plant, Tissue, Yeast, Enzymatic Reactions | |
| Not High-throughput Compatible (Manual) | |
| Mini | |
| Bacteria: ≤109 cells Blood: ≤0.2 mL Cells: ≤5 x 107 Plant: ≤250 mg Tissue: ≤200 mg Yeast: ≤5 x 108 cells Enzymatic reactions: ≤1.2 mL |
For Research Use Only. Not for use in diagnostic procedures.