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Thermo Scientific™ Miscela master per PCR Phusion™ Flash High-Fidelity
Thermo Scientific Phusion Flash PCR Master Mix was developed to save valuable laboratory time.
Marca: Thermo Scientific™ F548L
265.34 EUR valido fino al 2025-03-21
Usa il codice promo "24111" per avere il tuo prezzo promozionale.
Allerta:
To receive the discount customers must purchase three of the same product at list price in a single order to receive 33% discount. There is no limit to the multiples of 3 that customers can buy. Use promo code ”24111” to get your promotional price
Descrizione
Thermo Scientific Phusion Flash PCR Master Mix was developed to save valuable laboratory time. Ready-to-use 2X master mix preserves the fidelity and the yield in reactions when using very short PCR protocols. The unique master mix composition permits usage of short cycling protocols with both low and high complexity DNA templates (15 seconds per kilobase or less). The master mix utilizes Phusion Flash II DNA Polymerase, a modified proofreading DNA polymerase derived from Phusion Hot Start II High-Fidelity DNA Polymerase.
Features
- Extreme speed with extension times of 15 s/kb or less
- Hot start modification allows 'zero-time reactivation'
- Accurate proofreading DNA polymerase with a fidelity of 25X Taq
- High yields in reduced cycling time
- Minimized pipetting steps
Applications
- Fast PCR
- High-fidelity PCR
- Difficult (GC-rich) templates
- Template generation for sequencing Multiplex PCR
- Long-range PCR
- Cloning & mutagenesis
- Microarray
Notes
- Annealing rules for Phusion DNA Polymerases are different from many common DNA polymerases (such as Taq DNA polymerases).

Specifica
Phusion Flash High-Fidelity PCR Master Mix (5 x 1 mL) Store at -20°C. |
|
Phusion Flash II DNA Polymerase | |
High-Fidelity PCR Master Mix | |
Dry Ice | |
2X | |
Built-In Hot Start | |
Blunt | |
20 kb or less |
High | |
Fast | |
500 reazioni | |
Hot-start PCR, High-fidelity PCR | |
25X | |
500 Reactions | |
SuperMix or Master Mix | |
Colorless |
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For Research Use Only. Not for use in diagnostic procedures.