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Thermo Scientific™ DNA polimerasi Phusion High-Fidelity

Thermo Scientific Phusion High-Fidelity DNA polymerases set a gold standard for high performance PCR.

Marca:  Thermo Scientific™ F534L

297.00 EUR valido fino al 2024-11-30
Usa il codice promo "21651" per avere il tuo prezzo promozionale.

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Codice prodotto. 13585160

  • 540.00€ / 500 unità

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Includes:

Phusion High-Fidelity DNA Polymerase

  • Phusion DNA Polymerase 2 U/μL
  • 5X Phusion HF Buffer
  • 5X Phusion GC Buffer
  • DMSO
  • 50 mM MgCl2 solution

Both Phusion HF Buffer and Phusion GC Buffer provide 1.5 mM MgCl2 in the final 1X concentration.

Phusion Green High-Fidelity DNA Polymerase

  • Phusion DNA Polymerase 2 U/μL
  • 5X Phusion Green HF Buffer
  • 5X Phusion Green GC Buffer
  • DMSO
  • 50 mM MgCl2 solution

Both Phusion Green HF Buffer and Phusion Green GC Buffer provide 1.5 mM MgCl2 in the final 1X concentration.

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Descrizione

Descrizione

Thermo Scientific Phusion High-Fidelity DNA polymerases set a gold standard for high performance PCR. Featuring an error rate 50-fold lower than that of Taq and 6-fold lower than that of Pfu, Phusion High-Fidelity DNA Polymerase is an excellent choice for cloning and other applications requiring high fidelity. Phusion DNA polymerases offer robust performance with short protocol times, even in the presence of PCR inhibitors, and generate higher yields with lower enzyme amounts than other DNA polymerases.

Features of Phusion™ High-Fidelity DNA Polymerase include:

  • High fidelity (52X Taq)
  • Fast PCR due to short extension times (15–30 s/kb)
  • Robust performance, minimal optimization needed
  • High yields of PCR products with minimal enzyme amounts
  • Available in Green buffer format for direct loading of PCR products on gels (F-534S or F-534L)

Applications

  • High-fidelity PCR
  • Cloning
  • Template generation for sequencing
  • Amplification of difficult (GC-rich) templates
  • Long-range PCR (up to 20 kb)
  • Mutagenesis
  • High throughput PCR
  • Microarray

Note: Annealing rules for Phusion DNA polymerases are different from many common DNA polymerases (such as Taq DNA polymerases). For optimal results, use our convenient Tm calculator.

TRUSTED_SUSTAINABILITY
Specifica

Specifica

• Phusion DNA Polymerase, 2 U/μL
• 5X Phusion Green HF and GF Buffers
• DMSO
• 50 mM MgCl2

Phusion Green HF and Phusion Green GC Buffers each provide 1.5 mM MgCl2 in the final 1X concentration.
Store at -20°C.
Phusion High-Fidelity DNA Polymerase
High-Fidelity DNA Polymerase
Dry Ice
2 U/μL
No
Blunt
20 kb or less
High
Fast
500 U
Standard PCR, High-fidelity PCR
52X
500 Reactions
Standalone
Green
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For Research Use Only. Not for use in diagnostic procedures.