Thermo Scientific™ Pierce™ ChIP-grade Protein A/G Magnetic Beads

Highlights:

• Rapid – obtain purified DNA that is ready for quantitative PCR in about 8 hours

• Efficient and reproducible – micrococcal nuclease digestion and nuclear lysis are highly optimized

• Sensitive – obtain results with as few as 10,000 cells (1 x 10⁴)

• Low background – Pierce Protein A/G Magnetic Beads are blocked in a non-DNA-containing reagent to minimize background

• Complete – optimized positive control reagents are included: RNA polymerase II antibody and GAPDH promoter PCR primers

Includes:

Kit contains reagents for chromatin preparation, IP, DNA purification, and positive controls (antibody and primers)

Requires:

In vivo crosslinker (such as formaldehyde), micro-tip sonicator (such as Misonix™ Sonicator 3000), ChIP qualified antibody of choice, PCR primers for DNA sequence of interest, PCR master mix (containing a dye such as SYBR™ Green, if qPCR is desired), and a qPCR instrument

Recommended for:

• Determine sites of specific protein-DNA interactions on genomic DNA by PCR or ChIP-Seq
• Monitor the effects of histone modifications or chemical agents on DNA binding