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Thermo Scientific™ Kit di isolamento glicoproteine ConA e WGA Pierce™
Sfoglia Tutto Thermo Scientific ProdottiDescrizione
Thermo Scientific™ Pierce Glycoprotein Isolation Kits use ConA and WGA lectin affinity resins and optimized buffers to efficiently isolate glycoproteins from serum, tissue and cultured cell lysates, thus enabling downstream analysis.
Glycosylation is a post-translational modification that plays an important role in biological functions, including immune regulation, inflammation, cell-to-cell adhesion and cell signaling. Asparagine (N-linked) and serine/threonine residues (O-linked) are glycosylated during passage through the endoplasmic reticulum and golgi apparatus in eukaryotic and prokaryotic (i.e., Archaea and Eubacteria) systems. Glycoconjugates are important for immune regulation, inflammation, cell-to-cell adhesion and contact inhibition, cell signaling, protection against proteolytic degradation, and other biological processes.
Highlights:
High recovery – equivalent or greater glycoprotein recovery than the kits and lectin resins of other suppliers
Fast – glycoprotein purification in less than one hour
Versatile – isolate glycoproteins from various sample types; e.g., human serum and cell lysate
Robust – lectin does not leach from resin when processing sample
Convenient – complete kits include lectin resins and spin columns with all necessary reagents
Compatible with Bradford-based protein assays – dialysis or protein precipitation of recovered glycoproteins is not required prior to protein assay
Includes:
ConA or WGA beaded agarose resin (1.1mL), binding/wash buffer, elution buffer, and microcentrifuge spin columns and accessories.
Specifica
Specifica
| Descrizione | Kit di isolamento glicoproteine Pierce, WGA |
| Content And Storage | Sufficient For: Isolating glycoproteins with strong affinity for WGA from 10 samples of up to 640 μL (1 to 1.5 mg total protein) each • WGA Lectin Resin, 1.1 mL • Binding/Wash Buffer (5X), 6.5 mL • Elution Buffer, 5 mL • Spin Columns, 10 columns • Collection Tubes, 20 tubes Store at 4°C. |
| Product Line | Pierce |
| Quantità | 10 reactions kit |
| Bersaglio | Glycoprotein |
| Tipo | Glycoprotein Isolation Kit |
| Fase stazionaria | Lectin |
| Sufficiente per | 10 Reactions |
| Formato | Kit, Microcentrifuge, Spin Column |
| Tipo di colonna | Affinity, Agarose Resin |
For Research Use Only. Not for use in diagnostic procedures.
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