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Thermo Scientific™ Pierce™ Fab Micro Preparation Kit
Digest human or mouse IgG antibodies with immobilized papain protease and purify Fab fragments using Protein A agarose.
Marca: Thermo Scientific™ 44685
Descrizione
Thermo Scientific™ Pierce Fab Preparation Kits use immobilized papain protease to digest human or mouse IgG antibodies to make separate Fab and Fc fragments and subsequently to purify the Fab using Protein A agarose.
These kits use papain, a nonspecific thiol-endopeptidase, to enzymatically cleave whole IgG just above the hinge region to create two separate Fab fragments and one Fc fragment per antibody molecule. Because the papain protease is supplied in immobilized form as beaded agarose resin, the digestion reaction is easily stopped by removing the resin from the IgG solution; the result is digest products that are enzyme-free. The antibody digestion reaction is performed in convenient disposable spin columns that allow efficient removal of the immobilized protease and maximum recovery of the IgG fragments. Also included in the kits are Protein A spin columns and buffers to efficiently purify the resulting fragments. Protein A binds the Fc fragments and undigested IgG, allowing the pure Fab fragments to be recovered in the flow-through fraction. Thermo Scientific Zeba™ Desalting Spin Columns make IgG-sample preparation quick and easy.
Highlights:
Enzyme-free digestion products – immobilized papain (beaded agarose resin) provides for control of the digestion reaction and complete removal of resulting antibody fragments from the protease
Suitable for human and other species of IgG – the kit procedure is optimized for human, mouse and rabbit IgG, but papain-based digestion is effective for many species and subclasses of IgG including rat, goat and pig (Note: for best results with mouse IgG1, use Part No. 44980)
High capacity – kit provides for digestion and purification from as much as 4mg of IgG at a time. Use the standard size kit for fragmenting 0.25 to 4mg IgG, and the micro kit for 25 to 250μg IgG
Provides ready-to-use Fab – digestion and final recovery of purified Fab fragments occurs in neutral pH sodium phosphate buffer, suitable for storage or immediate use in typical applications
Complete and easy to use – kits include all reagents, spin columns and accessories needed to prepare and purify antibody fragments
Includes:
Papain agarose resin, cysteine, digestion buffer, Protein A columns with binding and elution buffers, spin desalting columns and accessories
Specifica
Pierce Fab Micro Preparation Kit | |
10 Antibody Samples, each containing 25 to 250 μg IgG | |
Pierce™ | |
Sufficient For: 10 antibody samples, each containing 25 to 250 μg IgG • Pierce Papain Agarose, 0.5 mL • Cysteine-HCl, 1 g • Fab Digestion Buffer, 55 mL • NAb Protein A Plus Spin Columns, 0.2 mL, 2 columns • PBS Packs (each makes 500 mL), 2 packs • IgG Elution Buffer, 50 mL • Zeba Desalt Spin Columns, 7K MWCO, 0.5 mL, 10 columns • Spin Columns, 0.8 mL, 10 columns • Microcentrifuge Tubes, 2 mL, 30 tubes Store at 4–8°C. |
Sicurezza e movimentazione
- Pierce Fab Micro Preparation Kit
Avvertenza
- Pericolo
Categoria di pericolo
- Lesione oculare grave/irritazione oculare Categoria 2
- Sensibilizzante delle vie respiratorie Categoria 1
- Corrosione/irritazione della pelle Categoria 2
- Tossicità specifica per organi bersaglio (esposizione singola) Categoria 3
Indicazioni di pericolo
- H315-Provoca irritazione cutanea.
- H319-Provoca grave irritazione oculare.
- H334-Può provocare sintomi allergici o asmatici o difficoltà respiratorie se inalato.
- H335-Può irritare le vie respiratorie.
Consigli di prudenza
- P261-Evitare di respirare la polvere/i fumi/i gas/la nebbia/i vapori/gli aerosol.
- P264-Lavare accuratamente dopo l'uso.
- P280-Indossare guanti/indumenti protettivi/Proteggere gli occhi/il viso.
- P284-¢Quando la ventilazione del locale è insufficiente! indossare un apparecchio di protezione respiratoria.
- P304+P340-IN CASO DI INALAZIONE: trasportare l'infortunato all'aria aperta e mantenerlo a riposo in posizione che favorisca la respirazione.
- P305+P351+P338-IN CASO DI CONTATTO CON GLI OCCHI: sciacquare accuratamente per parecchi minuti. Togliere le eventuali lenti a contatto se è agevole farlo. Continuare a sciacquare.
- P312-Contattare un CENTRO ANTIVELENI/un medico/in caso di malessere.
- P342+P311-In caso di sintomi respiratori: contattare un CENTRO ANTIVELENI/un medico/ .
- P501b-Smaltire il prodotto/recipiente in conformità con le disposizioni locali/regionali/nazionali/internazionali.
Informazioni supplementari
- MIXTURE LIST-Contiene: Sodium chloride, Sodium phosphate dibasic, Papain, L-(+)-Cysteine hydrochloridemonohydrate
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For Research Use Only. Not for use in diagnostic procedures.