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Thermo Scientific™ Tamponi per campione per la marcatura di corsia Pierce™
Ready-to-use 5X SDS-PAGE sample loading buffers available in reducing and nonreducing formulations, both with a pink dye buffer-front marker.
Marca: Thermo Scientific™ 39000
97.67 EUR valido fino al 2024-08-31
Usa il codice promo "21743" per avere il tuo prezzo promozionale.
Dettagli aggiuntivi : Peso : 1.81440kg
Descrizione
Thermo Scientific™ Pierce Lane Marker Sample Buffers are ready-to-use 5X SDS-PAGE sample loading buffers, available in reducing and nonreducing formulations, with a pink dye buffer-front marker.
Lane Marker Reducing and Non-Reducing Sample Buffers are convenient and ready to use for SDS-polyacrylamide gel electrophoresis (SDS-PAGE) and Western blotting. Formulated as 5X stocks rather than the traditional 2X stocks, they enable a larger volume of protein solution to be included in the sample that is loaded in each well. Particularly unique in these buffers is the use of a bright pink tracking dye instead of the traditional bromophenol blue dye. This pink dye marks not only the dye front in the gel but also transfers and permanently binds to nitrocellulose membranes. Consequently, the dye can be used to assess transfer efficiency and align cut membranes. The Reducing Sample Buffer contains DTT as the reducing agent, eliminating the repugnant odor associated with mercaptoethanol-containing buffers.Lane Marker Sample Buffers are easy to use. Simply mix one part Sample Buffer with four parts protein sample, heat denature, and then load the gel sample wells. Visualize progress of the electrophoresis run by monitoring the location of the pink dye front. Lane Marker Sample Buffers may be used in denaturing gels of all kinds and are compatible with coomassie dye and silver staining techniques, as well as Western blotting procedures.
Highlights:
Easy to see – bright pink dye marks the buffer front, clearly indicating the progress of the electrophoresis run
Concentrated – 5X stocks enable a greater range of protein sample volumes to be used for electrophoresis
Transferable marker dye – pink dye transfers with protein to nitrocellulose membrane, enabling verification of transfer direction and orientation of the blot
Flexibility in SDS-PAGE applications – available in two ready-to-use formulations for reducing or non-reducing denaturing SDS polyacrylamide electrophoresis
Specifica
Store single-use aliquots at -5 to -30°C. | |
Sample Loading Buffers | |
Fluorescence | |
Lane Marker Reducing Sample Buffer | |
Pierce™ |
Tampone riducente per campione per la marcatura di corsia (5X) Pierce | |
5X | |
SDS-PAGE | |
SDS-PAGE Gels | |
5 mL |
Sicurezza e movimentazione
- Pierce Lane Marker Reducing Sample Buffer
Avvertenza
- Attenzione
Categoria di pericolo
- Lesione oculare grave/irritazione oculare Categoria 2
Indicazioni di pericolo
- H319-Provoca grave irritazione oculare.
Consigli di prudenza
- P264-Lavare accuratamente dopo l'uso.
- P280-Indossare guanti/indumenti protettivi/Proteggere gli occhi/il viso.
- P303+P361+P353-IN CASO DI CONTATTO CON LA PELLE (o con i capelli): togliere immediatamente tutti gli indumenti contaminati. Sciacquare la pelle/fare una doccia.
Informazioni supplementari
- MIXTURE LIST-Contiene: glycerol, trometamol, sodium dodecyl sulphate
For Research Use Only. Not for use in diagnostic procedures.