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Thermo Scientific™ Kit di preparazione Pierce™ per IgG murine 1 Fab e F(ab')2

Codice prodotto. 10781775 Sfoglia Tutto Thermo Scientific Prodotti
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Quantità:
Kit resina da 2.5 ml
Dimensione della confezione:
1 set
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Codice prodotto. 10781775

Marca: Thermo Scientific™ 44980

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Descrizione

Efficiently digest mouse IgG1 antibodies into Fab or F(ab')2 fragments with immobilized ficin protease.

Thermo Scientific™ Pierce Mouse IgG1; Fab and F(ab')2 Preparation Kits use immobilized ficin protease to efficiently digest mouse IgG1 into Fab or F(ab')2 fragments, depending on the chosen reaction conditions.

The kits use ficin protease to enzymatically digest the Fc portion of whole IgG to yield Fab or F(ab')2 fragments. Because the ficin protease is supplied in immobilized form as beaded agarose resin, the digestion reaction is easily stopped by removing the resin from the IgG solution; the result is digest products that are enzyme-free. An optimized procedure and high-performance desalting and Protein A spin columns make these kits easy to use.

Highlights:

  • Enzyme-free digestion products – immobilized ficin (beaded agarose resin) provides for control of the digestion reaction and complete removal of resulting antibody fragments from the protease

  • Optimized for mouse IgG1 – the kit procedure is specifically optimized for monoclonal mouse IgG1 antibodies, providing the best possible yield for this antibody subclass

  • High capacity – kit provides for digestion and purification from as much as 4mg of IgG at a time. Use the standard size kit for fragmenting 0.25 to 4mg IgG, and the micro kit for 25 to 250μg IgG

  • Complete and easy to use – kits include all reagents, spin columns and accessories needed to prepare and purify antibody fragments

Includes:

Ficin agarose resin, cysteine, digestion buffer, Protein A columns with binding and elution buffers, spin desalting columns and accessories

TRUSTED_SUSTAINABILITY

Specifica

Quantità Kit resina da 2.5 ml
Product Line Pierce
Content And Storage Sufficient For: 10 antibody samples, each containing 0.25 to 4 mg IgG
• Pierce Ficin Agarose, 2.5 mL
• Cysteine-HCl, 1 g
• Mouse IgG1 Digestion Buffer, 120 mL
• NAb Protein A Plus Spin Column, 1 mL, 1 column
• Protein A Binding Buffer, 120 mL
• IgG Elution Buffer, 120 mL
• Zeba Desalt Spin Columns, 7K MWCO, 2 mL, 10 columns
• Spin Columns, 0.8 mL, 10 columns
• Microcentrifuge tubes, 2 mL, 30 tubes

Store at 4–8°C.

Safety and Handling

compliance-icons
Product Identifier
  • Pierce Mouse IgG1 Fab and F(ab')2 Preparation Kit
 Signal Word
  • Attenzione
 Hazard Category
  • Lesione oculare grave/irritazione oculare Categoria 2
  • Corrosione/irritazione della pelle Categoria 2
  • Tossicità specifica per organi bersaglio (esposizione singola) Categoria 3
 Hazard Statement
  • H315-Provoca irritazione cutanea.
  • H319-Provoca grave irritazione oculare.
  • H335-Può irritare le vie respiratorie.
 Precautionary Statement
  • P261-Evitare di respirare la polvere/i fumi/i gas/la nebbia/i vapori/gli aerosol.
  • P264-Lavare accuratamente dopo l'uso.
  • P280-Indossare guanti/indumenti protettivi/Proteggere gli occhi/il viso.
  • P304+P340-IN CASO DI INALAZIONE: trasportare l'infortunato all'aria aperta e mantenerlo a riposo in posizione che favorisca la respirazione.
  • P305+P351+P338-IN CASO DI CONTATTO CON GLI OCCHI: sciacquare accuratamente per parecchi minuti. Togliere le eventuali lenti a contatto se è agevole farlo. Continuare a sciacquare.
  • P312-Contattare un CENTRO ANTIVELENI/un medico/in caso di malessere.
  • P337+P313-Se l'irritazione degli occhi persiste, consultare un medico.
  • P501b-Smaltire il prodotto/recipiente in conformità con le disposizioni locali/regionali/nazionali/internazionali.
 Supplemental information
  • MIXTURE LIST-Contiene: Sodium chloride, L-(+)-Cysteine hydrochloride monohydrate

For Research Use Only. Not for use in diagnostic procedures.

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