Learn More
Thermo Scientific™ NBT Substrate Powder (nitro-blue tetrazolium chloride)
Powders and ready-made nitro-blue tetrazolium and 5-bromo-4-chloro-3'-indolyphosphate solutions for chromogenic detection of alkaline phosphatase probes.
Marca: Thermo Scientific™ 34035
196.19 EUR valido fino al 2024-11-29
Usa il codice promo "21683" per avere il tuo prezzo promozionale.
Descrizione
Thermo Scientific™ Pierce NBT and BCIP Substrates are powders and ready-made solutions of nitro-blue tetrazolium and 5-bromo-4-chloro-3'-indolyphosphate for chromogenic blot and IHC staining with alkaline phosphatase probes.
The combination of NBT (nitro-blue tetrazolium chloride) and BCIP (5-bromo-4-chloro-3'-indolyphosphate p-toluidine salt) yields an intense, insoluble black-purple precipitate when reacted with alkaline phosphatase, a popular enzyme conjugate for antibody probes. Pierce NBT and BCIP powders are available individually and in two convenient, ready-to-use solutions (with and without a levamisole suppressor of endogenous phosphatase activity).Highlights:
AP substrate – for detection of alkaline phosphatase (alk-phos) activity on solid media, including nitrocellulose and PVDF membranes and fixed tissue samples
Chromogenic – no special equipment needed for visualization; produces purple-black precipitate that is easy to photograph
Package options – choose individual NBT and BCIP powders, or preformulated, ready-to-use solutions with or without levamisole suppressor
1-Step™ NBT/BCIP
- Ideal for immunoblotting applications
- Minimal assay-to-assay variability
1-Step NBT/BCIP Plus Suppressor
- Ideal for staining tissues assayed with calf intestinal alkaline phosphatase without the interference caused by endogenous phosphatases
- Contains 1mM levamisole to inhibit endogenous phosphatase activity
Specifica
Pierce NBT Substrate | |
Colorimetric | |
Nitrocellulose, PVDF | |
Alkaline Phosphatase | |
Upon receipt store product at 4°C protected from moisture. Product is shipped at ambient temperature. |
Substrate | |
Powder | |
1 g | |
Alkaline Phosphatase |
Sicurezza e movimentazione
- NBT Substrate Powder (nitro-blue tetrazolium chloride)
Avvertenza
- Attenzione
Categoria di pericolo
- Tossicità acuta Categoria 4
- Lesione oculare grave/irritazione oculare Categoria 2
- Corrosione/irritazione della pelle Categoria 2
Indicazioni di pericolo
- H302-Nocivo se ingerito.
- H315-Provoca irritazione cutanea.
- H319-Provoca grave irritazione oculare.
Consigli di prudenza
- P260-Non respirare la polvere/i fumi/i gas/la nebbia/i vapori/gli aerosol.
- P280-Indossare guanti/indumenti protettivi/Proteggere gli occhi/il viso.
- P301+P310-IN CASO DI INGESTIONE: contattare immediatamente un CENTRO ANTIVELENI/un medico/
- P305+P351+P338-IN CASO DI CONTATTO CON GLI OCCHI: sciacquare accuratamente per parecchi minuti. Togliere le eventuali lenti a contatto se è agevole farlo. Continuare a sciacquare.
- P501b-Smaltire il prodotto/recipiente in conformità con le disposizioni locali/regionali/nazionali/internazionali.
For Research Use Only. Not for use in diagnostic procedures.