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Thermo Scientific™ RapidFinder™ Vegan ID Kit
Determine the presence of vertebrate animal DNA in food samples by simple and reliable Real Time-PCR detection to support vegan label claims.
Marca: Thermo Scientific™ A24412
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Descrizione
Simply and reliably detect the presence of animal DNA in food samples by real-time PCR using the RapidFinder™ Vegan ID Kit. Unlike ELISA based methods, when used with the GMO Extraction Kit (Cat. No. 4466336) for sample preparation, the RapidFinder Vegan ID Kit detects animal DNA in both raw and processed foods and provides a sensitivity down to 0.01% of animal DNA to support vegan label claims. The included positive control, which comprises 0.1% v/v of vertebrate animal DNA, can be used as a reference to set the threshold of detection of the assay. Invertebrate animal species such as seafood (crustaceans and molluscs) and terrestrial insects such as worms, crickets, and grasshoppers are not detected by the assay.
• Simple: Real-time PCR based assay, no electrophoresis needed
• Flexible: Vertebrate animal DNA detected in any food or feed sample, both raw and processed
• Reliable: Internal Positive Control (IPC) provides process-control check for PCR inhibitors
• Fast: From sample to result in the same day
How the RapidFinder™ Vegan ID Kit works
Animal DNA detection is accomplished by real-time PCR using two TaqMan™-MGB probes. The target sequence, detected in the FAM dye channel, specifically detects one mitochondrial DNA sequence common to all animal species. Also included in each reaction mix is an internal positive control detected in the VIC™ dye channel, which is used to verify the absence of PCR inhibitors.
By performing a separate reaction with the included positive control, users are able to verify the system and assay are working correctly. More importantly, it enables easy data interpretation. The positive control is provided as 0.1% vertebrate animal DNA, and by comparing sample results to the control results, a threshold of detection is provided.
Sample preparation
DNA sample preparation for the RapidFinder™ Vegan ID Kit workflow is accomplished with the easy-to-use GMO Extraction Kit (Cat. No. 4466336). Using 10 g of sample rather than the smaller sample sizes accommodated by most other DNA isolation procedures minimizes sampling errors. The GMO Extraction Kit is compatible with raw and processed foods, as well as composite food samples.
Part of a suite of solutions for meat species detection
The RapidFinder™ Vegan ID Kit is just one of our many kits available for the sensitive detection of various animal species in food and feed samples. Other RapidFinder™ animal species identification kits available include those for the detection of beef, goat, rabbit, water buffalo, duck, pork, horse, sheep, ruminant, chicken, turkey, and poultry.
The RapidFinder™ Animal Species Identification kits are made available through a partnership with Health in Code, S.L.
Specifica
| Contains 1 tube each of: Vegan Master Mix (store at -20°C), General Master Mix (store at -20°C upon receipt; 2-8 °C after initial use. Store protected from light), Positive Control (store at -20°C) |
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| Primer-probe | |
| qPCR | |
| Vegan Identification Kit | |
| 48 Reactions |
| RapidFinder Vegan ID Kit | |
| High | |
| Fast | |
| qPCR |
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