missing translation for 'onlineSavingsMsg'
Learn More
Learn More
Rat heart tissue lysate, Denatured; Abnova
Marca: Abnova L036W1.200ug
Questo articolo non è restituibile.
Consulta la politica di reso
Descrizione
Western Blotting
Specifica
| Western Blot | |
| Whole tissue lysate (denatured) | |
| Tissue lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined with Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The lysate was adjusted to 3.75 mg/ml, and then mixed with 5X Sample Buffer to become final 3 mg/ml in 1X Sample Buffer. The lysate was heated at 95°C for 5 min, and cooled rapidly. | |
| 200 μg | |
| Rat | |
| Store at -80°C. Aliquot to avoid repeated freezing and thawing. |
| Heart | |
| Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF. | |
| 12.5% SDS-PAGE Stained with Coomassie Blue. | |
| In ready-to-use 1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue). | |
| 3 mg/mL |
Product Content Correction
Your input is important to us. Please complete this form to provide feedback related to the content on this product.
Product Title
Spot an opportunity for improvement?Share a Content Correction