DiscoverX Ready-to-Assay™ ETA Endothelin Receptor Frozen Cells

Descrizione

The Ready-to-Assay™ ETA Endothelin Receptor Frozen Cells are a simple, thaw-and-assay cell format for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This assay assesses ligand (e.g. endothelin) based activation of EDNRA GPCR activity via detection of increasing levels of intracellular calcium. The included cells overexpress EDNRA alongside endogenous high levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. Upon activation of EDNRA , the resulting calcium activity can be fluorescently assayed. Ready-to-assay kits consist of two vials of the cells in a convenient thaw-and-use format.