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Applied Biosystems™ resDNASEQ™ Quantitative Pichia pastoris DNA Kits
The resDNASEQ™ Quantitative Pichia pastoris DNA Kit is a quantitative PCR (qPCR)-based system for the detection of host cell DNA from Pichia pastoris cells.
Marca: Applied Biosystems™ 4464340
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Descrizione
The resDNASEQ™ Quantitative Pichia pastoris DNA Kit is a quantitative PCR (qPCR)-based system for the detection of host cell DNA from Pichia pastoris cells, an expression system commonly used for the production of recombinant proteins. Reliable and rapid, the resDNASEQ™ system enables sensitive (as low as 15 pg DNA/mL test sample) and specific quantitation of Pichia pastoris cell DNA. This performance helps ensure a high degree of confidence in quantitation data obtained from a broad range of sample types.
Features of the resDNASEQ Quantitative Pichia pastoris DNA Kit include:• Highly sensitive quantitation using proven TaqMan™ real-time qPCR technology
• Consistent performance across the expected range of DNA fragment sizes
• Includes master mix, TaqMan™ primer/probe mix, and Pichia pastoris genomic DNA standard
This kit is available with or without sample preparation reagents in the form of a PrepSEQ™ Residual DNA Sample Preparation Kit, which is optimized for highly efficient residual DNA recovery from complex mixtures of proteins, buffers, and salts.
The resDNASEQ™ Quantitative Pichia pastoris DNA Kit is designed to provide highly sensitive detection of Pichia pastoris DNA, allowing you to use small sample volumes to generate accurate results. The broad linear range of TaqMan™ real-time qPCR technology allows you to test samples with variable levels of Pichia pastoris DNA in the same assay, such as in-process samples with higher amounts of DNA or bulk drug substance with very low amounts.
Easy to use
With the resDNASEQ™ Quantitative Pichia pastoris DNA Kit you can perform qPCR analysis to compare the amount of Pichia pastoris DNA in your test samples to a standard curve generated with known amounts of purified Pichia pastoris genomic DNA standard. The resDNASEQ™ kit includes almost everything you need to carry out these steps, from TaqMan™ primer/probe mix to Pichia pastoris genomic DNA standard, as well as step-by-step instructions.
Specific to Pichia pastoris DNA, no cross-reactivity with unrelated DNA
The target of the assay is highly specific—it only detects a Pichia pastoris-specific region of a multicopy genetic element. We selected this region using extensive bioinformatic analysis of multiple related and unrelated species.
Consistent performance even with fragmented DNA
For accurate quantitation of residual Pichia pastoris DNA, assay results must be unaffected by the size of the DNA molecules present in the test sample. With the resDNASEQ™ system you get consistent assay performance across a range of DNA fragment sizes.
Specifica
| resDNASEQ Pichia DNA Control • Pichia DNA Control, 30 ng/μL, 40 μL, store at –15 to –25°C • DNA Dilution Buffer (DDB), 7 mL, store at –15 to –25°C before first use and 2−8°C after first use resDNASEQ Pichia DNA Real-Time PCR Reagents • Environmental Master Mix (2X), 2 x 0.75 mL, store at –15 to –25°C before first use and 2−8°C after first use, protect from light • Pichia DNA Assay Mix (10X), 300 μL, store at –15 to –25°C, protect from light • Negative Control (water), 1.0 mL, store at –15 to –25°C before first use and 2−8°C after first use PrepSEQ Nucleic Acid Extraction Kit • Lysis Buffer (2 bottles, 50 mL/bottle) • Binding Solution/Isopropanol (1 empty bottle) • Wash Buffer Concentrate (2 bottles, 26 mL/bottle) • Elution Buffer (1 bottle, 25 mL) • Proteinase K Buffer (1 bottle, 50 mL) • Magnetic Particles (2 tubes, 1.5 mL/tube) • Proteinase K (20 mg/mL) (1 tube, 1.25 mL), store at or below –20°C • PK Buffer II (1 bottle, 11 mL) Store all at room temperature unless otherwise indicated. PrepSEQ Residual DNA Sample Preparation Kit • Proteinase K (20 mg/mL), 1 tube, 1.25 mL • Yeast tRNA (10 mg/mL), 1 tube, 0.5 mL • Glycogen (5 mg/mL), 2 tubes, 1.0 mL/tube Store all at or below –20°C. |
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| Kit | |
| Pichia | |
| resDNASEQ™ | |
| Assay Kit and Sample Preparation |
| Primer-probe | |
| qPCR | |
| FAM | |
| 100 Reactions |