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Applied Biosystems™ resDNASEQ™ Quantitative Sf9 and Baculovirus DNA Kits
The resDNASEQ Quantitative Sf9 and Baculovirus DNA Kit is a duplex quantitative PCR (qPCR)-based system for the detection of residual DNA from the Sf9 baculovirus platform.
Marca: Applied Biosystems™ A47405
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Descrizione
The resDNASEQ Quantitative Sf9 and Baculovirus DNA Kit is a duplex quantitative PCR (qPCR)-based system for the detection of residual DNA from the Sf9 baculovirus platform used in the development of gene therapy, cell-based vaccines, and similar biotherapeutics. Reliable and rapid, the resDNASEQ system enables sensitive and specific quantitation of Sf9 host cell DNA and baculovirus DNA. This performance helps ensure a high degree of confidence in quantitation data obtained from a broad range of sample types, from in-process samples with different sample matrices to purified final product.
Features of the resDNASEQ Quantitative Sf9 and Baculovirus DNA Kit include:• Duplex assay design to quantitate both Sf9 and baculovirus residual DNA
• Highly sensitive quantitation using proven TaqMan real-time qPCR technology
• Consistent performance across the expected range of DNA fragment sizes
• Includes master mix, TaqMan primer/probe mix, and Sf9 and baculovirus DNA standard
This kit is available with or without sample preparation reagents in the form of a PrepSEQ Residual DNA Sample Preparation Kit, which is optimized for highly efficient residual DNA recovery from complex mixtures of proteins, buffers, and salts.
Integrated workflow solution
The resDNASEQ Quantitative Sf9 and Baculovirus DNA Kit is part of an integrated workflow for impurity and contaminant testing during biopharmaceutical manufacturing. The kit is compatible with the PrepSEQ Residual DNA Sample Preparation Kit, which offers both a manual and automated sample preparation workflow. The resDNASEQ kit has been validated on the Applied Biosystems 7500 Fast Real-Time PCR System and the QuantStudio 5 Real-Time PCR System. Data analysis is streamlined using AccuSEQ Real-Time PCR Detection Software that provides accurate quantitation and security, audit, and e-signature capabilities to help enable 21 CFR Pt 11 compliance.
Easy to use
With the resDNASEQ Quantitative Sf9 and Baculovirus DNA Kit, you perform qPCR analysis to compare the amount of Sf9 and baculovirus DNA in your test samples to standard curves generated with known amounts of purified Sf9 and baculovirus DNA standard. The resDNASEQ kit includes almost everything you need to carry out these steps, from master mix to TaqMan primer/probe mix to DNA standard, as well as step-by-step instructions.
Consistent performance
For accurate quantitation of residual Sf9 and baculovirus DNA, assay results must be unaffected by the size of the DNA molecules present in the test sample. With the resDNASEQ system you get consistent assay performance across a range of DNA fragment sizes.
Specifica
| resDNASEQ Sf9 and Baculovirus DNA Control • Multiplex DNA Control (Sf9 DNA Control, 30 ng/μL; Baculovirus DNA Control, 30 ng/μL), 40 μL, store at –15° to –25°C • DNA Dilution Buffer (DDB), 7 mL, store at –15° to –25°C before first use and 2−8°C after first use resDNASEQ Real-Time PCR Reagents • 2X Environmental Master Mix, 2 x 0.75 mL, store at –15° to –25°C before first use and 2−8°C after first use, protect from light • 10X Sf9 + Baculovirus DNA Assay Mix , 300 μL, store at –15° to –25°C, protect from light • Negative Control (water), 1.0 mL, store at –15° to –25°C before first use and 2−8°C after first use PrepSEQ Nucleic Acid Extraction Kit • Lysis Buffer (2 bottles, 50 mL/bottle) • Binding Solution/Isopropanol (1 empty bottle) • Wash Buffer Concentrate (2 bottles, 26 mL/bottle) • Elution Buffer (1 bottle, 25 mL) • Proteinase K Buffer (1 bottle, 50 mL) • Magnetic Particles (2 tubes, 1.5 mL/tube) store at 2°C to 8°C • Proteinase K (20 mg/mL) (1 tube, 1.25 mL) store at or below –20°C • PK Buffer II (1 bottle, 11 mL) Store all at room temperature unless otherwise indicated. PrepSEQ Residual DNA Sample Preparation Kit • Proteinase K (20 mg/mL), 1 tube, 1.25 mL • Yeast tRNA (10 mg/mL), 1 tube, 0.5 mL • Glycogen (5 mg/mL), 2 tubes, 1.0 mL/tube Store all at or below –20°C. |
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| Kit | |
| SF9 + Bacoluvirus | |
| resDNASEQ™ | |
| Assay Kit and Sample Preparation |
| Primer-probe | |
| qPCR | |
| FAM + VIC | |
| 100 Reactions |