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IBA Lifesciences pDSG-IBA Small Mammalian Expression Vector
Designed for protein expression for mammalian HEK293E cells (MEXi 293E cells). IBA Lifesciences GmbH Small Mammalian Expression Vector is a small transient expression vector especially developed for the use in combination with the MEXi mammalian expression system.
Marca: IBA Lifesciences 5-5221-001
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Descrizione
pDSG-IBA vectors for MEXi mammalian expression system
- Small transient expression vector especially developed for the use in combination with the MEXi mammalian expression system.
- Designed for high-level stable and non-replicative transient expression in most mammalian hosts containing the following elements:
- Human cytomegalovirus (CMV) immediate-early promoter for high-level expression in a wide range of mammalian cells
- Episomal replication through Epstein Barr Virus replication origin (oriP) requires chromosomally expressed nuclear antigen encoded by EBNA-1
- Ampicillin resistance and ColE1 replication origin (pUC) for propagation in E. coliin a high plasmid copy number
Please note that cloning into pDSG-IBA Acceptor Vectors compulsorily requires the restriction enzyme Esp3I since no other MCS for the integration of a gene of interest is available. In addition to the direct cloning of the gene of interest into pDSG-IBA vectors with Esp3I, another option via a so-called Entry-Vector is possible.
Specifica
| Constitutive | |
| CMV | |
| Geneticin™ (G-418) | |
| Mammalian | |
| Direct Cloning | |
| Forward | |
| pDSG-IBA104 |
| Ampicillin | |
| Vector | |
| Recommended: 2°C to 8°C for frequent usage, -20°C for long-term storage | |
| Twin-Strep-tag | |
| 250 ng/μL | |
| 5 μg | |
| BM40 secretion signal |