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Thermo Scientific™ Kit di frazionamento subcellulare di proteine per cellule in coltura

Enrich for cultured cell-specific cytoplasmic, membrane, nuclear soluble, chromatin-bound and cytoskeletal proteins in less than 3 hours.

Marca:  Thermo Scientific™ 78840

435.94 EUR valido fino al 2024-12-31
Usa il codice promo "21651" per avere il tuo prezzo promozionale.


Codice prodotto. 10455924

  • 614.00€ / 1 set
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Descrizione

Descrizione

Perform segregation and enrichment of proteins from five different cellular compartments with Thermo Scientific™ Subcellular Protein Fractionation Kit for Cultured Cells.

The kit enables the stepwise separation and extraction of cytoplasmic, membrane, nuclear soluble, chromatin-bound and cytoskeletal proteins from mammalian cultured cells. Extracts obtained with the Subcellular Protein Fractionation Kit for Cultured Cells are compatible with a variety of downstream applications including Western blotting, protein assays, electrophoretic mobility shift assays and reporter-gene and enzyme-activity assays. Fractionation of subcellular proteins enables protein localization assessment and protein enrichment from specific cellular compartments. The Thermo Scientific Subcellular Protein Fractionation Kit includes a combination of reagents for stepwise lysis of cells into functional cytoplasmic, membrane, nuclear soluble, chromatin-bound and cytoskeletal protein fractions in less than 3 hours. Extracts from each subcellular compartment generally have less than 15% contamination between fractions, which is sufficient purity for most experiments studying protein localization and redistribution.

Highlights:

  • Concise – extract functional cytoplasmic, membrane, nuclear soluble, chromatin-bound and cytoskeletal protein fractions in less than three hours

  • Convenient – perform a simple procedure without using ultracentrifugation over gradients

  • Complete – obtain cytoplasmic, membrane, soluble nuclear, chromatin-bound and cytoskeletal protein fractions from a single kit

  • Cultured Cells – developed and optimized for use with cultured mammalian cells; for tissue samples use the Subcellular Fractionation Kit for Tissues, Part No. 87790

  • Compatible – use with a variety of downstream applications including Western blotting, protein assays, electrophoretic mobility shift assays and reporter-gene and enzyme-activity assays

  • Small-scale – kit provides sufficient reagents to fractionate 50 cell pellets of 20μL each, equivalent to a total of 2g of cell paste or tissue

Includes:

Four extraction buffers, micrococcal nuclease enzyme, calcium chloride solution and protease inhibitor cocktail

Recommended for:

Extraction and enrichment of proteins from different cellular compartments; Separation and profiling of protein from the cytoplasm, nucleus, membrane and cytoskeleton from the same cell sample; Studying protein translocation

TRUSTED_SUSTAINABILITY
Specifica

Specifica

Subcellular Protein Fractionation Kit
Liquid
Cell Lysis Buffer, Detergent Solution, Subcellular Fractionation
1 Kit
Upon receipt store kit at -20°C, or store individual components as indicated above. Kit is shipped with dry ice.
Lysis Buffer
50 Samples
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Identificativo prodotto
  • Subcellular Protein Fractionation Kit for Cultured Cells

Avvertenza
  • Attenzione

Categoria di pericolo
  • Lesione oculare grave/irritazione oculare Categoria 2
  • Corrosione/irritazione della pelle Categoria 2

Indicazioni di pericolo
  • H315-Provoca irritazione cutanea.
  • H319-Provoca grave irritazione oculare.

Consigli di prudenza
  • P264-Lavare accuratamente dopo l'uso.
  • P280-Indossare guanti/indumenti protettivi/Proteggere gli occhi/il viso.
  • P305+P351+P338-IN CASO DI CONTATTO CON GLI OCCHI: sciacquare accuratamente per parecchi minuti. Togliere le eventuali lenti a contatto se è agevole farlo. Continuare a sciacquare.

Informazioni supplementari
  • MIXTURE LIST-Contiene: 4-(2-aminoethyl)-Benzenesulfonyl fluoride hydrochloride

SDS
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