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Thermo Scientific™ Kit di frazionamento subcellulare di proteine per cellule in coltura
Enrich for cultured cell-specific cytoplasmic, membrane, nuclear soluble, chromatin-bound and cytoskeletal proteins in less than 3 hours.
Marca: Thermo Scientific™ 78840
429.80 EUR valido fino al 2024-09-27
Usa il codice promo "21650" per avere il tuo prezzo promozionale.
Dettagli aggiuntivi : Peso : 1.36080kg
Descrizione
Perform segregation and enrichment of proteins from five different cellular compartments with Thermo Scientific™ Subcellular Protein Fractionation Kit for Cultured Cells.
The kit enables the stepwise separation and extraction of cytoplasmic, membrane, nuclear soluble, chromatin-bound and cytoskeletal proteins from mammalian cultured cells. Extracts obtained with the Subcellular Protein Fractionation Kit for Cultured Cells are compatible with a variety of downstream applications including Western blotting, protein assays, electrophoretic mobility shift assays and reporter-gene and enzyme-activity assays. Fractionation of subcellular proteins enables protein localization assessment and protein enrichment from specific cellular compartments. The Thermo Scientific Subcellular Protein Fractionation Kit includes a combination of reagents for stepwise lysis of cells into functional cytoplasmic, membrane, nuclear soluble, chromatin-bound and cytoskeletal protein fractions in less than 3 hours. Extracts from each subcellular compartment generally have less than 15% contamination between fractions, which is sufficient purity for most experiments studying protein localization and redistribution.Highlights:
Concise – extract functional cytoplasmic, membrane, nuclear soluble, chromatin-bound and cytoskeletal protein fractions in less than three hours
Convenient – perform a simple procedure without using ultracentrifugation over gradients
Complete – obtain cytoplasmic, membrane, soluble nuclear, chromatin-bound and cytoskeletal protein fractions from a single kit
Cultured Cells – developed and optimized for use with cultured mammalian cells; for tissue samples use the Subcellular Fractionation Kit for Tissues, Part No. 87790
Compatible – use with a variety of downstream applications including Western blotting, protein assays, electrophoretic mobility shift assays and reporter-gene and enzyme-activity assays
Small-scale – kit provides sufficient reagents to fractionate 50 cell pellets of 20μL each, equivalent to a total of 2g of cell paste or tissue
Includes:
Four extraction buffers, micrococcal nuclease enzyme, calcium chloride solution and protease inhibitor cocktail
Recommended for:
Extraction and enrichment of proteins from different cellular compartments; Separation and profiling of protein from the cytoplasm, nucleus, membrane and cytoskeleton from the same cell sample; Studying protein translocation
Specifica
Subcellular Protein Fractionation Kit | |
Liquid | |
Cell Lysis Buffer, Detergent Solution, Subcellular Fractionation | |
1 Kit |
Upon receipt store kit at -20°C, or store individual components as indicated above. Kit is shipped with dry ice. | |
Lysis Buffer | |
50 Samples |
Sicurezza e movimentazione
- Subcellular Protein Fractionation Kit for Cultured Cells
Avvertenza
- Attenzione
Categoria di pericolo
- Lesione oculare grave/irritazione oculare Categoria 2
- Corrosione/irritazione della pelle Categoria 2
Indicazioni di pericolo
- H315-Provoca irritazione cutanea.
- H319-Provoca grave irritazione oculare.
Consigli di prudenza
- P264-Lavare accuratamente dopo l'uso.
- P280-Indossare guanti/indumenti protettivi/Proteggere gli occhi/il viso.
- P305+P351+P338-IN CASO DI CONTATTO CON GLI OCCHI: sciacquare accuratamente per parecchi minuti. Togliere le eventuali lenti a contatto se è agevole farlo. Continuare a sciacquare.
Informazioni supplementari
- MIXTURE LIST-Contiene: 4-(2-aminoethyl)-Benzenesulfonyl fluoride hydrochloride
For Research Use Only. Not for use in diagnostic procedures.