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  8. 15252606

Thermo Scientific™ Miscela master per PCR Phire™ Tissue Direct

Thermo Scientific Phire Tissue Direct PCR Master Mix has been developed for amplification of DNA directly from a wide variety of tissues obtained from mice, human, fish, birds and insects.

Marca:  Thermo Scientific™ F170L

490.60 EUR valido fino al 2024-11-30
Usa il codice promo "21651" per avere il tuo prezzo promozionale.

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Codice prodotto. 15252606

  • 892.00€ / 500 reazioni

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Descrizione

Descrizione

Thermo Scientific Phire Tissue Direct PCR Master Mix has been developed for amplification of DNA directly from a wide variety of tissues obtained from mice, humans, fish, birds, and insects. The Master Mix containing Phire Hot Start II DNA Polymerase is specially formulated to perform PCR in the presence of different animal tissue–derived inhibitors such as collagen, melanin and eumelanin (hair, skin), and myoglobin (muscle). The kit also includes DNARelease Additive, which can be used to improve the release of DNA from difficult tissues.

Features

  • Direct PCR reduces time-consuming and expensive DNA purification steps
  • Available Dilution & Storage protocol allows multiple PCR reactions from a single sample
  • Specially engineered Phire DNA polymerase for extremely short PCR protocol times
  • Master mix format with premixed gel loading dye minimizes cross-contamination, reduces sample handling, and allows direct loading on gel

Applications

  • Genotyping
  • Transgene detection
  • Knockout analysis
  • Sequencing

Notes

  • The optimal annealing temperature for Phire DNA Polymerases may differ significantly from that of Taq-based polymerases.
TRUSTED_SUSTAINABILITY
Specifica

Specifica

• 2X Phire Tissue Direct PCR Master Mix, 10 x 1.25 mL
• Dilution Buffer, 2 x 5 mL
• DNARelease Additive, 1.3 mL
• 25 μM Control Primer Mix, 40 μL
• Nuclease-free water, 10 x 1.25 mL
• 50 μg O'GeneRuler Express DNA Ladder

Store at -20°C.
Direct PCR
Fast
Direct PCR Master Mix
Genotyping, Hot-start PCR
Built-In Hot Start
Blunt
Tissue
Medium
Phire Hot Start II DNA Polymerase
Yes
Wet or Dry Ice
2X
1250 Reactions
SuperMix or Master Mix
DNA
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Suggerimenti di prodotto

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For Research Use Only. Not for use in diagnostic procedures.