Thermo Scientific™ Verso 1-Step RT-PCR Kit ReddyMix, with ThermoPrime Taq

Descrizione

Thermo Scientific Verso 1-Step RT-PCR Kit ReddyMix with ThermoPrime Taq is a complete set of RT-PCR reagents that combines everything needed for RT-PCR. The Verso RT enzyme delivers high processivity and can be used at temperatures up to 57°C, delivering effective transcription through difficult RNA secondary structures. Verso RT also shows a better dynamic range than standard AMV or M-MuLV enzymes, with increases in template concentration giving rise to corresponding increases in product generated over a wider range of starting material concentrations.

An optional RT enhancer is provided with the kit and can be used during the RT step to remove any DNA contamination, eliminating the need for DNase I treatment.

This Verso 1-step RT-PCR system comes with the ReddyMix option, where the master mix contains a dye and precipitant that allow RT-PCR reactions to be loaded directly onto an electrophoresis gel. The red dye migrates between bromophenol blue and xylene cyanol at approximately 300 bp, depending on agarose concentration.

Features of the Verso 1-Step RT-PCR Kit ReddyMix with ThermoPrime Taq include:
• ReddyMix formulation—eliminates post-PCR steps required for electrophoresis
• Eliminates the need for DNase treatments—RT Enhancer (optional) prevents gDNA carry-over
• RT and PCR steps performed in single sealed tube. providing convenience and reduced contamination risk
• Verso RT enzyme is highly sensitive with a broad dynamic range—accurately detect RNA input from 1 pg to 1 μg
• Wide working temperature range (42–57°C) for success with GC-rich and other difficult templates
• RNase Inhibitor included in RT enzyme mix

Applications
• Gene expression analysis
• Viral/bacterial detection
• 1-step RT-PCR
• High throughput applications

Related products
Verso 1-Step RT-PCR Kit ReddyMix, with ThermoPrime Taq (Cat. No. AB1454LDA)
Verso 1-Step RT-PCR Kit, with Thermo-Start Taq (Hot Start) (Cat. No. AB1455A)