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Thermo Scientific™ Fosfatasi alcalina termosensibile FastAP
Catalyze the removal of 5'- and 3'-phosphate groups from DNA, RNA, nucleotides, and proteins using this thermosensitive alkaline phosphatase .
Marca: Thermo Scientific™ EF0651
65.66 EUR valido fino al 2025-03-21
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Descrizione
FastAP Thermosensitive Alkaline Phosphatase catalyzes the release of 5'- and 3'-phosphate groups from DNA, RNA, and nucleotides. This enzyme also removes phosphate groups from proteins.
FastAP is a novel alkaline phosphatase, which is active in all Thermo Scientific restriction enzyme buffers as well as in PCR buffers. It dephosphorylates all types of DNA ends (blunt, 5'- and 3'-overhangs) in 10 minutes at 37°C. The enzyme is inactivated in 5 minutes at 75°C . Therefore, removal of alkaline phosphatase is not required prior to ligation.
Highlights
- Recombinant enzyme
- Fast dephosphorylation – 10 minutes at 37°C
- Fast and complete inactivation – 5 minutes at 75°C
- Simultaneous digestion and dephosphorylation of vector DNA
- 100% active in restriction enzyme and PCR buffers
- One protocol for all types of DNA ends:
- 5’-overhangs
- 3’-overhangs
- blunt-ends
- single nucleotides
- PCR clean-up in conjunction with Exo I
- Protein dephosphorylation
Applications
- Dephosphorylation of cloning vector DNA to prevent recircularization during ligation
- Simultaneous digestion and dephosphorylation of vector DNA
- PCR product clean-up: nucleotide degradation prior to sequencing of PCR product
- Dephosphorylation of nucleic acid 5'-termini prior to labeling with T4 Polynucleotide Kinase
- Other applications where dephosphorylation of DNA and RNA substrates is necessary
- Protein dephosphorylation
Note
- Binding of FastAP Thermosensitive Alkaline Phosphatase to DNA may result in a band shift in agarose gels. To avoid this, incubate samples with 6X DNA Loading Dye & SDS Solution at 65°C for 10 minutes, and chill on ice prior to electrophoresis.
- FastAP Thermosensitive Alkaline Phosphatase is active in all restriction enzyme buffers and may be added directly to digested DNA. Heat inactivation of the restriction enzyme before dephosphorylation reaction is not necessary.

Specifica
1 U/μL | |
PCR Buffer, Enzyme Buffer | |
Alkaline Phosphatase |
Phosphatase | |
1000U |
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Certificati
Per poter ottenere i certificati è necessario indicare un numero di lotto. Per individuare il numero di lotto su ordini precedenti usi l'area dedicata allo stato degli ordini.
Numero di lotto | Tipo di certificato | Data | Catalog Number |
---|---|---|---|
2852097 | Certificato di analisi | 31/01/2025 | EF0651 |
2795893 | Certificato di analisi | 29/01/2025 | EF0651 |
2822095 | Certificato di analisi | 29/01/2025 | EF0651 |
2822096 | Certificato di analisi | 29/01/2025 | EF0651 |
2837045 | Certificato di analisi | 29/01/2025 | EF0651 |
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For Research Use Only. Not for use in diagnostic procedures.