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Cytiva Ficoll PM400
Polimeri di saccarosio ad alto peso molecolare per effetto della copolimerizzazione del saccarosio con l'epicloridrina. CytivaTM; Ficoll PM400 è un polimero di saccarosio sintetico neutro, altamente ramificato e idrofilo utilizzato per produrre gradienti di densità per la separazione di cellule e componenti subcellulari tramite centrifugazione o sedimentazione per gravità.
Marca: Cytiva 17-0300-10
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Descrizione
- For producing density gradients for separation of cells and subcellular components by centrifugation or by sedimentation at unit gravity
- Neutral, highly branched, hydrophilic polymer of sucrose which dissolves readily in aqueous solution
- Concentrations of up to 50% (w/v) covering densities of up to 1.2g/mL can be achieved
- Has better osmotic properties than sucrose
- Preserves functional and morphological integrity
- Useful for separating cells that are sensitive to centrifugation and for separating cells of similar density but different size
- Does not penetrate biological membranes
- Serves as the raw material for preparation of Ficoll-Paque gradients
- Used in other applications such as electrophoresis, hybridization, cryopreservation, and as a hapten carrier
- Supplied as dry powders
- Used in the formation of density gradients for the separation and isolation of eukaryotic cells, organelles, and bacterial cells, as a stabilizing agent and as a separation medium for the isolation of lymphocytes
- Used in defined culture media, nucleic acid hybridization, electrophoresis, and immunological studies
- Used for gradient centrifugation in all types of centrifuge rotors and for separation at unit gravity
Specifica
| 100 g | |
| Producono gradienti di densità per separare celle, componenti subcellulari | |
| 0.12 EU/mg | |
| Centrifugation Media | |
| Powder |
| Da 4° a 30 °C | |
| ≈17 ml/g | |
| White | |
| 1.2 g/ml |