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Gibco™ DMEM, high glucose, pyruvate

DMEM (Dulbecco's Modified Eagle Medium) is a widely used basal medium for supporting the growth of many different mammalian cells.

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Specifica

Linea cellulare HeLa, 293, Cos-7, and PC-12
Tipo di cellula Primary Fibroblasts, Neurons, Glial Cells, HUVECs, Smooth Muscle Cells
Classificazione Animal Origin-free
Concentrazione 1 X
Forma Liquid
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Requires: Supplementation


Descrizione

Descrizione

DMEM (Dulbecco's Modified Eagle Medium) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12. We offer a variety of DMEM modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

This DMEM is modified as follows:
With: High Glucose, L-glutamine, Phenol Red, Sodium Pyruvate
Without: HEPES

The complete formulation is available.

Using DMEM
DMEM is unique from other media as it contains 4 times the concentration of amino acids and vitamins than the original Eagle's Minimal Essential Medium. DMEM was originally formulated with low glucose (1 g/L) and sodium pyruvate, but is often used with higher glucose levels, with or without sodium pyruvate. DMEM contains no proteins, lipids, or growth factors. Therefore, DMEM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). DMEM uses a sodium bicarbonate buffer system (3.7 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.

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Shipping Condition: Room Temperature

Specifica

Specifica

HeLa, 293, Cos-7, and PC-12
Animal Origin-free
Liquid
High Glucose, Glutamine, Phenol Red, Sodium Pyruvate
cGMP-compliant under the ISO 13485 standard
Primary Fibroblasts, Neurons, Glial Cells, HUVECs, Smooth Muscle Cells
1 X
Sterile-filtered
No HEPES
Ambient
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missing translation for 'shelfLife' : 12 Months From Date of Manufacture

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For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.