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  7. Gibco™ Ham's F-12 Nutrient Mix

Gibco™ Ham's F-12 Nutrient Mix

Ham's F-12 Nutrient Mixture (F-12) was designed for serum-free, single-cell plating of Chinese Hamster Ovary (CHO) cells.

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Specifica

Linea cellulare CHO, COS-7, and rat prostate epithelial cells
Tipo di cellula Primary Rat Astrocytes
Classificazione Animal Origin-free
Concentrazione 1 X
Forma Liquid
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Requires: Supplementation


Descrizione

Descrizione

Ham's F-12 Nutrient Mixture (F-12) was designed for serum-free, single-cell plating of Chinese Hamster Ovary (CHO) cells. F-12 has since been used for serum-free growth of CHO cultures as well as serum-supplemented growth of other mammalian cells, including chondrocytes and rat prostate epithelial cells. We offer a variety of F-12 modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

This Ham's F-12 is modified as follows:

With: L-glutamine, Phenol Red

The complete formulation is available.

Using F-12

Compared to other basal media, F-12 contains a wider variety of components, including zinc, putrescine, hypoxanthine, and thymidine. F-12 contains no proteins or growth factors. Therefore, F-12 requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). F-12 uses a sodium bicarbonate buffer system (1.176 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.

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Shipping Condition: Room Temperature

Specifica

Specifica

CHO, COS-7, and rat prostate epithelial cells
Animal Origin-free
Liquid
Sterile-filtered
Glutamine, Phenol Red, Sodium Pyruvate
cGMP-compliant under the ISO 13485 standard
Primary Rat Astrocytes
1 X
Ham's F-12 Nutrient Mixture
Sterile-filtered
No HEPES
Gibco™
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Gibco™ Ham's F-12 Nutrient Mix

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