missing translation for 'onlineSavingsMsg'
Learn More

Invitrogen™ LentiArray™ Human Transcription Factor CRISPR Library

Targets 1,817 genes with up to 4 gRNA per gene target (pooled in a single well) for a total of 7,268 gRNAs

Marca:  Invitrogen™ A31938

Codice prodotto. 17138715

Questo articolo non è attualmente disponibile o è stato dismesso.
La preghiamo di consultare la pagina del prodotto per individuare le possibili alternative.
Visualizzare prodotti alternativi »

Esplora altre offerte speciali
Questo articolo non è restituibile. Consulta la politica di reso

Descrizione

Descrizione

  • The award-winning Invitrogen™ LentiArray™ Human Transcription Factor Library targets 1,817 genes with up to 4 gRNA per gene target (pooled in a single well) for a total of 7,268 gRNAs. Libraries are delivered as 200 μL of ready to use lentiviral particles per gene target with an average titer of 1x108 TU/mL (functional titer determined by antibiotic resistance)
  • Transcription factors are the key regulators of gene expression. The gene targets within this library were selected using the most up-to-date genome databases, including the NCBI RefSeq database, and cross-referenced to the Gene Ontology Consortium (GO) database and/or the HUGO Gene Nomenclature Committee (HGNC).
  • LentiArray CRISPR libraries are constructed using gRNA designs produced using Thermo Fisher Scientific’s proprietary CRISPR gRNA design algorithm. This algorithm incorporates the latest gRNA design research, as well as our extensive in-house experience, to produce the highest quality designs. The gRNA designs included in the LentiArray CRISPR libraries are selected for maximal editing efficiency and specificity and are designed to knock out all known isoforms of the target gene. For each gene target, up to four high-quality gRNAs are included to help ensure highly efficient knockout of the target gene in a wide array of cell types.
  • LentiArray CRISPR libraries are designed and constructed to give you complete control over your experimental design. They utilize a two-vector design, expressing the Cas9 nuclease and the gRNA off of separate lentiviral constructs, enabling you to dictate when and how the genome editing tools are delivered to your cells.
  • The LentiArray Cas9 lentivirus construct expresses a human, codon-optimized Cas9 nuclease with a blasticidin-resistance gene under the control of the EF-1α (EFS) promoter. You can choose to co-infect your cells with both the LentiArray gRNA lentiviruses and the Cas9 lentivirus or just the LentiArray Cas9 lentivirus to establish a stable Cas9 expressing cell line. With LentiArray CRISPR libraries you have the freedom to design the experimental approach that best suits your model system and your screening goals.
  • Furthermore, to minimize restrictions to your experimental design, the gRNA lentiviruses that make up the LentiArray CRISPR libraries do not contain fluorescent markers. This allows reporters of any wavelength to be used with the libraries and expands your ability to perform multiplexed experiments. For challenging cell lines, the LentiArray gRNA lentiviruses contain a puromycin resistance gene that allows enrichment of the population of edited cells and drives stronger phenotypes.Optimize your assay design and have full confidence in your results with LentiArray controls
  • High quality controls are the cornerstone of a successful screen.
TRUSTED_SUSTAINABILITY
Specifica

Specifica

1 library set, 200 μL/target.
Store at -68 to -85°C.
Genome Editing
Crispr Library
1 Library
96-well Plate
Human
Lentiviral
LentiArray™
U6
Dry Ice
gRNA
Suggerimenti di prodotto

Suggerimenti di prodotto

Videos
SDS
Documenti

Documenti

Certificati
Offerte speciali

Offerte speciali

Correzione del contenuto del prodotto

Fornite il vostro feedback sul contenuto del prodotto compilando il modulo sottostante.

Titolo del prodotto

Facendo clic su Invia, l'utente riconosce che potrebbe essere contattato da Fisher Scientific in merito al feedback fornito in questo modulo. Non condivideremo le vostre informazioni per altri scopi. Tutte le informazioni di contatto fornite saranno conservate in conformità con la nostra Politica sulla privacy. Informativa sulla privacy.

Grazie per averci aiutato a migliorare il nostro sito web. Il vostro feedback è stato inviato

For Research Use Only. Not for use in diagnostic procedures.