missing translation for 'onlineSavingsMsg'
Learn More
Learn More
Thermo Scientific™ LightShift™ Chemiluminescent RNA EMSA Kit
Study RNA-protein interactions with this non-radioactive EMSA kit for gel shift assays.
Marca: Thermo Scientific™ 20158
723.90 EUR valido fino al 2025-12-31
Usa il codice promo "24344" per avere il tuo prezzo promozionale.
Questo articolo non è restituibile.
Consulta la politica di reso
Descrizione
Thermo Scientific™ LightShift Chemiluminescent RNA EMSA Kit provides a non-radioactive solution for studying RNA-protein interactions using an electrophoretic mobility-shift assay (EMSA).
The RNA EMSA Kit uses biotinylated RNA probes and a chemiluminescent substrate system to achieve fast and safe detection of RNA-protein complexes with sensitivity equivalent to traditional 32P-isotopic methods. The complete kit comes with all reagents required to set up and optimize protein-RNA binding conditions, a positive control for protein-RNA interactions and reagents for chemiluminescent detection of the nucleic acid interaction.Highlights:
Sensitive – chemiluminescent detection comparable to radioactive detection
Time-saving – develop X-ray films after 1- to 5-minute exposures, versus 16 hour exposures needed with radioactive systems
Flexible – compatible with RNA probes biotinylated by different methods
Easy to use – complete kit includes optimized reagents for binding reactions and RNA probe detection
Non-radioactive – eliminate waste concerns from radioactive RNA probes
Specifica
| EMSA Assay | |
| Chemiluminescence | |
| Protein | |
| 100 reactions | |
| LightShift Chemiluminescent RNA EMSA Kit | |
| Not Target-Specific |
| Sufficient For: 100 binding reactions and detection on 1000 cm2 of membrane (10 mini-gel blots)• 125 nM Biotinylated IRE RNA Control 35 μL (store at -20°C) • 10 μM Unlabeled IRE RNA Control, 25 μL (store at -20°C) • 2 mg/mL Cytosolic Liver Extract, 50 μL (store at -20°C) • 10 mg/mL tRNA, 100 μL (store at -20°C) • 10X REMSA Binding Buffer, 1 mL (store at -20°C) • 50% Glycerol, 500 μL (store at -20°C) • 2M KCl, 500 μL (store at -20°C) • 1M MgCl2, 500 μL (store at -20°C) • DTT, lyophilized (store at -20°C) • Nuclease-Free Water, 1.5 mL (store at -20°C) • 5X REMSA Loading Buffer, 1 mL (store at -20°C) • Stabilized Streptavidin-HRP, 1.5 mL (store at 4°C) • Luminol/Enhancer Solution, 80 mL (store at 4°C) • Stable Peroxide Solution, 80 mL (store at 4°C) • Nucleic Acid Detection Blocking Buffer, 500 mL (store at 4°C) • 4X Wash Buffer, 500 mL (store at 4°C) • Substrate Equilibration Buffer, 500 mL (store at 4°C) |
|
| Kit | |
| myECL™ Imager, X-Ray Film | |
| LightShift™ | |
| 100 reactions | |
| Enhanced Chemiluminescence, Membrane Blot, Gel Shift |
Correzione del contenuto del prodotto
Fornite il vostro feedback sul contenuto del prodotto compilando il modulo sottostante.
Titolo del prodotto
For Research Use Only. Not for use in diagnostic procedures.
Individuate un'opportunità di miglioramento?Condividi una correzione di contenuto