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IBA Lifesciences pCSG-IBA Mammalian Expression Vector with Secretion
Designed for high-level episomal expression in mammalian hosts under G418 selection. Mammalian Expression Vector with Secretion is a large expression vector with universal features for transient expression as well as for generation of stable cell lines (NeoR).
Marca: IBA Lifesciences 5-5001-001
Descrizione
pCSG-IBA mammalian expression vectors- Designed for high-level episomal expression in mammalian hosts under G418 selection containing the following elements:
- Human cytomegalovirus (CMV) immediate-early promoter for high-level expression in a wide range of mammalian cells
- Episomal replication through Epstein Barr Virus replication origin (oriP) and nuclear antigen encoded by EBNA-1 in human, primate and canine cells and through SV40 replication origin in cells latently infected with SV40 or that express the SV40 large T antigen (e.g. HEK293T, COS-1, COS-7)
- Stable cell lines can be selected by the neomycin resistance gene (NeoR).
- In addition, the human cytomegalovirus (CMV) immediate-early promoter enables a high-level expression in a wide range of mammalian cells.
Please note that cloning into pCSG-IBA Acceptor Vectors compulsorily requires the restriction enzyme Esp3I since no other MCS for the integration of a gene of interest is available. Besides to the direct cloning of the gene of interest into pCSG-IBA vectors with Esp3I, another option via a so-called entry-vector is possible.
Specifica
Constitutive | |
CMV | |
Geneticin™ (G-418) | |
Untagged | |
pCSG-IBAwt2 |
Ampicillin | |
Vector | |
Recommended: 2°C to 8°C for frequent usage, -20°C for long-term storage | |
5 μg | |
Episomal expression (oriP, EBNA-1); BM40 secretion signal |
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