IBA Lifesciences MHC I-Strep H-2 Kb; Ovalbumin (SIINFEKL)

IBA Lifesciences GmbH MHC I-Strep H-2 Kb Ovalbumin SIINFEKL is for fluorescent staining/FACS as well as for magnetic isolation of antigen-specific (CD8+) T cells.

Marca: IBA Lifesciences 6-7015-001

Codice prodotto. 15623908

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Descrizione

MHC I-Strep H-2 Kb; Ovalbumin (SIINFEKL) is a recombinantly expressed MHC class I molecule that presents a peptide from a Ovalbumin.

Our MHC I Streptamer™ approach is based on our Strep-tag technology and facilitates reversible antigen-specific T cell labeling.

A Twin-Strep-tag™ fused to the MHC I-Strep permits multimerization of MHC I complexes on a Strep-Tactin™ backbone, resulting in the formation of MHC I Streptamers.

For multimerizing MHC I-Streps, Strep-Tactin™ TACS Agarose columns, Strep-Tactin™ Magnetic Microbeads or fluorophore-conjugated Strep-Tactin™ can be used.

This allows affinity chromatographic or magnetic isolation or cell staining for flow cytometry analysis and sorting (FACS), depending on the experimental requirements.

The addition of biotin reverses the multimerization of MHC class I molecules, leading to their dissociation from the target cells.

The resulting label-free cells are highly viable and fully functional.

Specifications

Capacità (sistema metrico)	40 μL
Contenuto kit	Fluorescent reagent for staining, magnetic T cell labeling and isolation
Quantità	40 μL

Product Suggestions Documents

Documents

Product Information

Manuals

Manual PR22 MHC I Streptamer 6-7015-001 Dimensione file: 1.2 MB

Methods & Protocols

Short Protocol PR38 MHC-I Staining 6-7015-001 Dimensione file: 1.1 MB

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