Perform high-purity, large-scale purification of GST-tagged proteins with glutathione that has been immobilized to a crosslinked 6% beaded agarose.
Marca: Thermo Scientific™ 25239
Perform high-purity, large-scale purification of GST-tagged proteins with Thermo Scientific™ Pierce Glutathione Superflow Agarose, which consists of glutathione that has been immobilized to a crosslinked 6% beaded agarose.
Additional Information and Online Ordering
Pierce Glutathione Superflow Agarose is a highly crosslinked, durable resin that does not compress at flow rates required for medium- to large-scale FPLC purifications. The resin holds up well to a variety of chemical and pH values, and it is compatible with common clean-in-place procedures. The specific binding of glutathione to the GST-tag results in high purity and high yield in a single step purification process.Highlights:
High dynamic binding capacity – binds 10mg of pure GST per mL of resin at a linear flow rate of 150cm/hour; can bind greater than 20mg/mL at 30cm/hour flow rate
High purity – provides greater than 90% purity when used to purify overexpressed GST or GST-fusion proteins from lysates
Robust – highly crosslinked 6% agarose Superflow beaded support tolerates linear flow rates up to 1200cm/hour
Compatible – maintains function after exposure to a wide variety of chemicals and pH values
Cost effective – resin is stable through multiple cycles of cleaning and reuse
Recommended for:
Medium- to large-scale FPLC purification of GST-tagged proteins
| Pierce Glutathione Superflow Agarose | |
| Glutathione onhighly crosslinked 6% beaded agarose (superflow 6), slurried in water with sodium azide | |
| 1 L | |
| Binding up to 30mg GST/mL resin (static capacity) or up to 10mg GST/mL resin (dynamic capacity) |
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For Research Use Only. Not for use in diagnostic procedures.
