Isotype mouse monoclonal antibody using an antigen-independent, microplate-based method that enables multiple samples to be tested in about one hour.
Marca: Thermo Scientific™ 37503
642.20 EUR valido fino al 2025-05-31
Usa il codice promo "25977" per avere il tuo prezzo promozionale.
Thermo Scientific™ Pierce Rapid ELISA Mouse Antibody Isotyping Kit provides antigen-independent determination of mouse monoclonal antibody isotype using a microplate method that enables multiple samples to be tested in about one hour.
The kit enables easy identification of mouse immunoglobulin class, subclass and light chain composition. This fast assay uses ELISA strip-plates pre-coated in different wells with anti-mouse heavy-chain capture antibodies (anti-IgG1, IgG2a, IgG2b, IgG3, IgA and IgM) or anti-mouse light-chain capture antibody (kappa or lambda). This approach eliminates the need to purify and immobilize an antigen to determine immunoglobulin isotype. A mouse monoclonal antibody sample applied to the wells can be isotyped within one hour. Results are evaluated qualitatively by visual inspection or quantitatively by measuring the absorbance at 450nm.Highlights:
Fast – determine antibody isotype in approx. 1 hour; considerably faster than traditional ELISA kits
Convenient – eight-well strip format allows partial use of the plate; use one strip (column) for each sample (12 samples per plate)
Specific – characterize antibodies for six different subclasses and two different light-chain types
Sensitive – accurate characterization with samples containing more than 3ng/mL of test antibody
Flexible – kit is compatible with hybridoma cell culture supernatant, ascites fluid or purified antibodies
Cost effective – cost per assay is less than with single-use assay strips or cassettes
No special equipment needed – assess results visually or measure quantitatively using an ordinary ELISA plate reader (450nm)
Complete and easy to use – includes pre-coated plates, detecting antibody, buffers, and TMB substrate and stop solutions
Includes:
Precoated 96-well Isotyping plates, 5 plates; Goat anti-Mouse IgG+IgA+IgM-HRP Conjugate, 30mL; TMB Substrate, 55mL; Stop solution, 55mL; 30X Wash buffer, 200mL; Tris buffered saline, 2 packs
| Sufficient For: 60 isotype determinations (8 wells per test sample) • Pre-coated 96-well Isotyping Plates, 5 plates • Goat Anti-Mouse IgG+IgA+IgM-HRP Conjugate, 30 mL • TMB Substrate, 55 mL • Stop Solution, 55 mL • 30X Wash Buffer, 200 mL • TBS Buffer Packs (each makes 500 mL), 2 packs Store at 4°C. |
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| Microplate Reader | |
| Isotyping Assay | |
| Pierce™ |
| ELISA (Antigen Capture), Absorbance | |
| 60 Tests | |
| Colorimetric | |
| Target-specific |
Per poter ottenere i certificati è necessario indicare un numero di lotto. Per individuare il numero di lotto su ordini precedenti usi l'area dedicata allo stato degli ordini.
| Numero di lotto | Tipo di certificato | Data | Catalog Number |
|---|---|---|---|
| 429609-003 | Certificato di analisi | 14/04/2025 | |
| 429609-002 | Certificato di analisi | 01/11/2024 | 37503 |
| 429609-001 | Certificato di analisi | 01/11/2024 | 37503 |
| 390528-009 | Certificato di analisi | 01/11/2024 | 37503 |
| 390528-008 | Certificato di analisi | 30/08/2024 | 37503 |
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For Research Use Only. Not for use in diagnostic procedures.
