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Thermo Scientific™ Yeast beta-Galactosidase Assay Kit

Extract protein from yeast two-hybrid reporter systems and measure beta-galactosidase activity using compatible Y-PER Reagent and b-Gal Assay Reagent.

Marca:  Thermo Scientific™ 75768

Codice prodotto. 10581664

  • 259.00€ / 1 set

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Descrizione

Descrizione

Thermo Scientific™ Yeast β-Galactosidase Assay Kit is specially formulated for yeast cells and ideal for identifying protein interactions in vivo using two-hybrid systems.

The Yeast β-Galactosidase Assay Kit allows for qualitative or quantitative determination of β-galactosidase activity in solution directly from colonies growing on solid medium. A portion of the colony is suspended in a mixture of Thermo Scientific Y-PER™ Yeast Protein Extraction Reagent and the β-Galactosidase Assay Buffer. After a brief incubation period, the solution turns yellow from the hydrolysis of o-nitrophenyl-β-D-galactopyranoside (ONPG) to o-nitrophenol (ONP) and galactose in a mildly alkaline solution. The assay becomes quantitative if calibrated to cell number by measuring the optical density at 660nm.

Highlights:

  • Efficient lysis of yeast cells and a colorimetric detection system
  • Quantitative or qualitative assay for yeast colonies grown on solid media or in suspension
  • Colorimetric assay easily measured in a plate reader or spectrophotometer (420nm)
  • Adaptable to 96-well microplates or tube assay formats
  • Perform homogeneous screening assays or measure activity secondarily after cell lysis
  • Also compatible with prepared lysates from other species such as bacteria

Includes:

Thermo Scientific Y-PER Yeast Protein Extraction Reagent (Part No. 78990), 2X Assay Buffer and Stop Solution

TRUSTED_SUSTAINABILITY
Specifica

Specifica

Sufficient For: 250 colonies or 250 culture samples (100 μL each)
  • Y-PER Yeast Protein Extraction Reagent, 25 mL (store at room temperature)
  • 2X beta-Galactosidase Assay Buffer, 25 mL (store at -20°C)
  • 1M Sodium Carbonate Stop Solution, 25 mL (store at room temperature)
    		•
    Absorbance
    Yeast beta-Galactosidase Assay Kit
    96-well Plate
    75 mL
    Beta-Gal Substrate
    Beta-Galactosidase
    Reporter Enzyme
    Microplate Reader
    Enzyme Labeled
    Pierce™
    ONPG
    250 colonies or 250 culture samples (100 μL each)
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