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Applied Biosystems™ resDNASEQ™ Human Residual DNA Quantitation Kits
The resDNASEQ Human Residual DNA Quantitation Kit is a quantitative PCR (qPCR)-based system for the detection of host cell DNA from human cell lines used as expression systems for recombinant proteins.
Marca: Applied Biosystems™ A27335
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Descrizione
The resDNASEQ Human Residual DNA Quantitation Kit is a quantitative PCR (qPCR)-based system for the detection of host cell DNA from human cell lines used as expression systems for recombinant proteins. Reliable and rapid, the resDNASEQ system enables sensitive (as low as 15 pg DNA/mL test sample) and specific quantitation of human cell DNA. This performance helps ensure a high degree of confidence in quantitation data obtained from a broad range of sample types, from in-process samples to final product, whether the sample contains high molecular weight or sheared DNA.
Features of the resDNASEQ Human Residual DNA Quantitation Kit include:• Highly sensitive quantitation using proven TaqMan real-time qPCR technology
• Consistent performance across the expected range of DNA fragment sizes
• Includes master mix, TaqMan primer/probe mix, and human genomic DNA standard
This kit is available with or without sample preparation reagents in the form of a PrepSEQ Residual DNA Sample Preparation Kit, which is optimized for highly efficient residual DNA recovery from complex mixtures of proteins, buffers, and salts.
The resDNASEQ Human Residual DNA Quantitation Kit provides highly sensitive detection of human DNA, allowing you to use small sample volumes to generate accurate results. The broad linear range of TaqMan real-time qPCR technology allows you to test samples with variable levels of human DNA in the same assay, such as in-process samples with higher amounts of DNA or bulk drug substance with very low amounts.
Easy to use
With the resDNASEQ Human Residual DNA Quantitation Kit you perform qPCR analysis to compare the amount of human DNA in your test samples to a standard curve generated with known amounts of purified human genomic DNA standard. The resDNASEQ kit includes almost everything you need to carry out these steps, from master mix to TaqMan primer/probe mix to human genomic DNA standard, as well as step-by-step instructions.
Consistent performance
For accurate quantitation of residual human DNA, assay results must be unaffected by the size of the DNA molecules present in the test sample. With the resDNASEQ system you get consistent assay performance across a range of DNA fragment sizes.
*Due to the high degree of similarity between genomes, DNA from closely-related primate species such as monkey (Vero), chimpanzee, and orangutan are expected be detected by the resDNASEQ Human Quantitation Kit.
Specifica
| resDNASEQ Human DNA Control • Human DNA Control, 30 ng/μL, 40 μL, store at –15 to –25°C • DNA Dilution Buffer (DDB), 7 mL, store at –15 to –25°C before first use and 2−8°C after first use resDNASEQ Human DNA Real-Time PCR Reagents • Environmental Master Mix (2X), 2 x 0.75 mL, store at –15 to –25°C before first use and 2−8°C after first use, protect from light • Human DNA Assay Mix (10X), 300 μL, store at –15 to –25°C, protect from light • Negative Control (water), 1.0 mL, store at –15 to –25°C before first use and 2−8°C after first use PrepSEQ Nucleic Acid Extraction Kit • Lysis Buffer (2 bottles, 50 mL/bottle) • Binding Solution/Isopropanol (1 empty bottle) • Wash Buffer Concentrate (2 bottles, 26 mL/bottle) • Elution Buffer (1 bottle, 25 mL) • Proteinase K Buffer (1 bottle, 50 mL) • Magnetic Particles (2 tubes, 1.5 mL/tube) • Proteinase K (20 mg/mL) (1 tube, 1.25 mL), store at or below –20°C • PK Buffer II (1 bottle, 11 mL) Store all at room temperature unless otherwise indicated. PrepSEQ Residual DNA Sample Preparation Kit • Proteinase K (20 mg/mL), 1 tube, 1.25 mL • Yeast tRNA (10 mg/mL), 1 tube, 0.5 mL • Glycogen (5 mg/mL), 2 tubes, 1.0 mL/tube Store all at or below –20°C. |
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| Kit | |
| Human DNA | |
| resDNASEQ™ | |
| Assay Kit and Sample Preparation |
| Primer-probe | |
| qPCR | |
| FAM | |
| 100 Reactions |